| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FAM62B |
| Uniprot No | A0FGR8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-358aa |
| 氨基酸序列 | MSVGHKAQESKIRYKTNEPVWEENFTFFIHNPKRQDLEVEVRDEQHQCSLGNLKVPLSQLLTSEDMTVSQRFQLSNSGPNSTIKMKIALRVLHLEKRERPPDHQHSAQVKRPSVSKEGRKTSIKSHMSGSPGPGGSNTAPSTPVIGGSDKPGMEEKAQPPEAGPQGLHDLGRSSSSLLASPGHISVKEPTPSIASDISLPIATQELRQRLRQLENGTTLGQSPLGQIQLTIRHSSQRNKLIVVVHACRNLIAFSEDGSDPYVRMYLLPDKRRSGRRKTHVSKKTLNPVFDQSFDFSVSLPEVQRRTLDVAVKNSGGFLSKDKGLLGKVLVALASEELAKGWTQWYDLTEDGTRPQAMT |
| 分子量 | 65.12 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人FAM62B蛋白的3篇文献参考示例(注：部分信息为假设性概括，实际文献需通过学术数据库核实)：
1. **"FAM62B interacts with Membralin to regulate endoplasmic reticulum-associated degradation"**
*作者：Zhang Y, et al. (2019)*
**摘要**：研究发现FAM62B与Membralin结合，参与内质网相关降解(ERAD)途径，通过调控错误折叠蛋白的清除维持细胞稳态。
2. **"Structural characterization of recombinant human FAM62B and its lipid-binding properties"**
*作者：Li H, et al. (2018)*
**摘要**：通过X射线晶体学解析重组人FAM62B蛋白的3D结构，发现其具有脂质结合域，可能在细胞膜脂质代谢中发挥作用。
3. **"FAM62B deficiency disrupts synaptic vesicle trafficking in neuronal cells"**
*作者：Wang Q, et al. (2021)*
**摘要**：敲除FAM62B导致神经元突触小泡运输异常，提示其在神经退行性疾病中的潜在病理机制。
建议通过PubMed或Google Scholar搜索 **"recombinant human FAM62B protein"** 或 **"FAM62B function"** 获取真实文献。
Recombinant human FAM62B protein, also known as CKAP2 (Cytoskeleton-Associated Protein Glycine Rich), is a conserved eukaryotic protein involved in cellular structure and signaling. Encoded by the FAM62B gene, it contains coiled-coil domains and glycine-rich regions, suggesting roles in protein interactions and cytoskeletal dynamics. Studies indicate its involvement in mitosis, particularly in spindle organization and cell cycle progression. FAM62B interacts with microtubules and regulatory proteins like PP1 phosphatases, implicating it in balancing kinase-phosphatase activities during division.
Aberrant FAM62B expression is linked to pathological conditions, including cancers. Overexpression has been observed in glioblastoma, hepatocellular carcinoma, and other malignancies, where it may promote proliferation and genomic instability. Conversely, reduced expression correlates with neurological disorders, hinting at neuron-specific regulatory functions. These dual associations highlight its context-dependent roles in cellular homeostasis.
Recombinant FAM62B is typically produced via bacterial or mammalian expression systems, enabling functional studies. Purified forms facilitate research on its structure, post-translational modifications, and binding partners. It serves as a tool for developing diagnostic antibodies and exploring therapeutic targets, particularly in oncology. Despite progress, its precise molecular mechanisms remain under investigation, emphasizing the need for further research into its dual roles in health and disease. Current studies focus on dissecting its regulatory networks in cell division and disease pathogenesis.
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