关键信息
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基因名
PAPOLB
- 应用
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别名
PAPOLB; PAPTPoly(A) polymerase beta; PAP-beta; EC 2.7.7.19; Polynucleotide adenylyltransferase beta; Testis-specific poly(A) polymerase
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9NRJ5
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表达区间
1-636 aa
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氨基酸序列
MPFPVTTQGP PQPAPPPNRY GVSSPISLAV PKETDCLLTQ RLIETLRPFG VFEEEEELQR RILVLEKLNN LVKEWIREIS ESKSLPQSVI ENVGGKIFTF GSYRLGVHTK GADIDALCVA PSHVDRSDFF TSFYAKLKLQ EEVKDLRAVE EAFVPVIKLC FDGIEIDILF ARLALQTIPE DLDLRDDSLL KNLDIRCIRS LNGCRVTDEI LHLVPNIDNF RLTLRAIKLW AKCHNIYSNI LGFLGGVSWA MLVARTCQLY PNAVASTLVR KFFLVFSEWE WPNPVLLKEP EERNLNLPVW DPRVNPSDRY HLMPIITPAY PQQNSTYNVS ISTRMVMIEE FKQGLAITHE ILLSKAEWSK LFEAPSFFQK YKHYIVLLAS ASTEKQHLEW VGLVESKIRI LVGSLEKNEF ITLAHVNPQS FPAPKENPDM EEFRTMWVIG LGLKKPDNSE ILSIDLTYDI QSFTDTVYRQ AVNSKMFEMG MKITAMHLRR KELHQLLPHH VLQDKKAHST EGRRLTDLND SSFDLSAGCE NSMSVPSSTS TMKTGPLISS SQGRNSPALA VMTASVANIQ ATEFSLQQVN TNESSGVALN ESIPHAVSQP AISPSPKAMV ARVVSSTCLI SHPDLQETQQ QTYLIL
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分子量
71.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
PAPOLB, or poly(A) polymerase beta, is an enzyme that plays a pivotal role in RNA metabolism, particularly in the post-transcriptional modification of RNA molecules. Research into PAPOLB has gained significance due to its involvement in various cellular processes, including mRNA stability, polyadenylation, and gene expression regulation. Abnormalities in PAPOLB function have been linked to several diseases, including cancer and neurodegenerative disorders, highlighting its potential as a therapeutic target. The enzyme's dual function—supporting both polyadenylation and poly(A) tail remodeling—has drawn attention to its mechanistic pathways and regulatory networks. Furthermore, recent advances in proteomics and molecular biology have facilitated deeper investigations into PAPOLB's structure-function relationships, offering insights into its role in RNA processing and cellular responses to stress. Understanding PAPOLB's intricate functions and interactions within the cellular milieu is crucial for developing innovative strategies to manipulate its activity for therapeutic interventions, making it a prominent focus in the fields of molecular biology and biotechnology.
