| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EMID2 |
| Uniprot No | Q96A83 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 21-441aa |
| 氨基酸序列 | TGFLYPFSAA ALQQHGYPEP GAGSPGSGYA SRRHWCHHTV TRTVSCQVQN GSETVVQRVY QSCRWPGPCA NLVSYRTLIR PTYRVSYRTV TVLEWRCCPG FTGSNCDEEC MNCTRLSDMS ERLTTLEAKV LLLEAAERPS SPDNDLPAPE STPPTWNEDF LPDAIPLAHP VPRQRRPTGP AGPPGQTGPP GPAGPPGSKG DRGQTGEKGP AGPPGLLGPP GPRGLPGEMG RPGPPGPPGP AGNPGPSPNS PQGALYSLQP PTDKDNGDSR LASAIVDTVL AGVPGPRGPP GPPGPPGPRG PPGPPGTPGS QGLAGERGTV GPSGEPGVKG EEGEKAATAE GEGVQQLREA LKILAERVLI LEHMIGIHDP LASPEGGSGQ DAALRANLKM KRGGAQPDGV LAALLGPDPG QKSVDQASSR K |
| 分子量 | 45.38 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人EMID2蛋白的虚构参考文献及其摘要(基于类似研究主题的常见方向)：
1. **文献名称**：*Expression and Purification of Recombinant Human EMID2 in Mammalian Cells*
**作者**：Smith A, et al.
**摘要**：研究通过哺乳动物表达系统高效表达重组人EMID2蛋白，优化纯化流程，并通过质谱验证其正确折叠及糖基化修饰，为功能研究提供高纯度蛋白。
2. **文献名称**：*EMID2 Regulates Extracellular Matrix Assembly in Osteoarthritis*
**作者**：Chen L, et al.
**摘要**：探讨重组人EMID2蛋白在骨关节炎中的功能，发现其通过调控胶原纤维组装抑制软骨降解，提示其作为潜在治疗靶点。
3. **文献名称**：*Structural Characterization of Recombinant EMID2 and Its Role in Tumor Angiogenesis*
**作者**：Yamamoto K, et al.
**摘要**：解析重组EMID2蛋白的晶体结构，揭示其通过整合素信号通路抑制肿瘤血管生成，为癌症治疗提供新策略。
4. **文献名称**：*Functional Analysis of EMID2 Mutants in Hereditary Kidney Disease*
**作者**：Gupta R, et al.
**摘要**：利用重组EMID2蛋白分析基因突变对蛋白功能的影响，发现特定突变导致细胞外基质异常，与遗传性肾病的病理机制相关。
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**说明**：上述文献为示例性质，实际研究中建议通过PubMed或Web of Science搜索关键词(如"EMID2 recombinant"或"EMID2 function")获取真实文献。关注近年研究可聚焦EMID2在组织纤维化、癌症或发育生物学中的角色。
Recombinant human EMID2 (Emilin-2) protein is a engineered form of the extracellular matrix (ECM) protein EMID2. which belongs to the EMILIN family characterized by cysteine-rich EMI domains and involved in ECM organization and cell-ECM interactions. EMID2. also known as EMILIN-2. plays a critical role in tissue homeostasis, vascular development, and wound healing by regulating collagen fibrillogenesis, cell adhesion, and integrin-mediated signaling pathways. Structurally, it contains an N-terminal EMI domain for protein-protein interactions, a central collagen-binding domain, and a C-terminal coiled-coil region. Dysregulation of EMID2 has been associated with cancer progression, fibrosis, and cardiovascular diseases, where it exhibits context-dependent roles in tumor suppression or metastasis promotion.
The recombinant protein is typically produced in mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications and functional activity. Purified recombinant EMID2 enables mechanistic studies of its interaction with α4/α9β1 integrins and collagen types I/IV, as well as its regulatory effects on TGF-β signaling and matrix metalloproteinase (MMP) activity. It serves as a vital tool for investigating ECM remodeling processes and developing therapeutic strategies targeting tissue repair or fibrotic disorders. Current challenges include optimizing its solubility and stability due to the inherent structural complexity of multidomain ECM proteins.
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