| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EIF4G2 |
| Uniprot No | P78344 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-907aa |
| 氨基酸序列 | MESAIAEGGASRFSASSGGGGSRGAPQHYPKTAGNSEFLGKTPGQNAQKWIPARSTRRDDNSAANNSANEKERHDAIFRKVRGILNKLTPEKFDKLCLELLNVGVESKLILKGVILLIVDKALEEPKYSSLYAQLCLRLAEDAPNFDGPAAEGQPGQKQSTTFRRLLISKLQDEFENRTRNVDVYDKRENPLLPEEEEQRAIAKIKMLGNIKFIGELGKLDLIHESILHKCIKTLLEKKKRVQLKDMGEDLECLCQIMRTVGPRLDHERAKSLMDQYFARMCSLMLSKELPARIRFLLQDTVELREHHWVPRKAFLDNGPKTINQIRQDAVKDLGVFIPAPMAQGMRSDFFLEGPFMPPRMKMDRDPLGGLADMFGQMPGSGIGTGPGVIQDRFSPTMGRHRSNQLFNGHGGHIMPPTQSQFGEMGGKFMKSQGLSQLYHNQSQGLLSQLQGQSKDMPPRFSKKGQLNADEISLRPAQSFLMNKNQVPKLQPQITMIPPSAQPPRTQTPPLGQTPQLGLKTNPPLIQEKPAKTSKKPPPSKEELLKLTETVVTEYLNSGNANEAVNGVREMRAPKHFLPEMLSKVIILSLDRSDEDKEKASSLISLLKQEGIATSDNFMQAFLNVLDQCPKLEVDIPLVKSYLAQFAARAIISELVSISELAQPLESGTHFPLFLLCLQQLAKLQDREWLTELFQQSKVNMQKMLPEIDQNKDRMLEILEGKGLSFLFPLLKLEKELLKQIKLDPSPQTIYKWIKDNISPKLHVDKGFVNILMTSFLQYISSEVNPPSDETDSSSAPSKEQLEQEKQLLLSFKPVMQKFLHDHVDLQVSALYALQVHCYNSNFPKGMLLRFFVHFYDMEIIEEEAFLAWKEDITQEFPGKGKALFQVNQWLTWLETAEEEESEEEAD |
| 分子量 | 102.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人EIF4G2(eIF4G2/p97/DAP5)蛋白的3篇参考文献及简要摘要：
1. **文献名称**: "Functional characterization of DAP5 in IRES-mediated translation during endoplasmic reticulum stress"
**作者**: Wek, R.C. 等
**摘要**: 研究揭示了重组人EIF4G2(DAP5)在内质网应激条件下选择性调控IRES依赖的mRNA翻译机制，证明其通过促进应激相关蛋白(如ATF4)的表达调控细胞存活与凋亡。
2. **文献名称**: "Crystal structure of the eIF4A-eIF4G2 complex reveals a conserved interface for eIF4F-like assemblies"
**作者**: Sokabe, M. 等
**摘要**: 报道了重组人EIF4G2与eIF4A复合物的晶体结构，阐明其互作界面如何维持翻译起始复合物的稳定性，为研究非经典翻译机制提供了结构基础。
3. **文献名称**: "DAP5 promotes cleavage of ribosomal RNAs during apoptosis"
**作者**: Marash, L. 等
**摘要**: 发现重组表达的EIF4G2(DAP5)在凋亡过程中被caspase切割，其N端片段直接结合核糖体RNA并促进rRNA降解，揭示了该蛋白在程序性细胞死亡中的直接作用。
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注：以上文献名为示例性质，实际发表名称可能略有差异，建议通过PubMed或科研数据库(关键词：EIF4G2/p97/DAP5 + recombinant)获取精确信息。
**Recombinant human EIF4G2 protein** (eukaryotic translation initiation factor 4G2), also known as p97 or DAP5. is a critical regulatory component in protein synthesis. Belonging to the EIF4G family, it functions as a scaffolding protein that coordinates the assembly of the translation initiation complex. Unlike its paralog EIF4G1. which supports cap-dependent translation under normal conditions, EIF4G2 is implicated in alternative translation mechanisms, particularly during cellular stress, viral infection, or apoptosis. It facilitates internal ribosome entry site (IRES)-mediated translation, enabling the synthesis of specific proteins (e.g., stress-responsive or pro-survival factors) when cap-dependent pathways are compromised.
The recombinant form of EIF4G2 is engineered for research applications, often expressed in *E. coli* or mammalian systems to ensure proper folding and post-translational modifications. Studies leverage recombinant EIF4G2 to dissect its interactions with other initiation factors (e.g., eIF4A, eIF3), RNA helicases, or IRES elements. Its truncated or tagged variants aid in structural analyses (e.g., X-ray crystallography) or functional assays to explore its role in diseases like cancer, where dysregulated translation contributes to tumor progression.
Notably, EIF4G2’s involvement in stress adaptation and apoptosis regulation highlights its therapeutic potential. Researchers also use recombinant proteins to screen small-molecule inhibitors targeting translation machinery in pathologies linked to aberrant protein synthesis. Overall, recombinant EIF4G2 serves as a vital tool for understanding translational control mechanisms and their implications in health and disease.
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