| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EIF3S7 |
| Uniprot No | O15371 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-548aa |
| 氨基酸序列 | MAKFMTPVIQDNPSGWGPCAVPEQFRDMPYQPFSKGDRLGKVADWTGATYQDKRYTNKYSSQFGGGSQYAYFHEEDESSFQLVDTARTQKTAYQRNRMRFAQRNLRRDKDRRNMLQFNLQILPKSAKQKERERIRLQKKFQKQFGVRQKWDQKSQKPRDSSVEVRSDWEVKEEMDFPQLMKMRYLEVSEPQDIECCGALEYYDKAFDRITTRSEKPLRSIKRIFHTVTTTDDPVIRKLAKTQGNVFATDAILATLMSCTRSVYSWDIVVQRVGSKLFFDKRDNSDFDLLTVSETANEPPQDEGNSFNSPRNLAMEATYINHNFSQQCLRMGKERYNFPNPNPFVEDDMDKNEIASVAYRYRRWKLGDDIDLIVRCEHDGVMTGANGEVSFINIKTLNEWDSRHCNGVDWRQKLDSQRGAVIATELKNNSYKLARWTCCALLAGSEYLKLGYVSRYHVKDSSRHVILGTQQFKPNEFASQINLSVENAWGILRCVIDICMKLEEGKYLILKDPNKQVIRVYSLPDGTFSSDEDEEEEEEEEEEEEEEET |
| 分子量 | 85.91 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人EIF3S7蛋白的3篇参考文献的简要列举(因实际研究文献不足，以下为模拟示例)：
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1. **文献名称**：*Structural Insights into the Human EIF3S7 Subunit and Its Role in Translation Initiation Complex Assembly*
**作者**：Smith A et al.
**摘要**：通过X射线晶体学解析重组人EIF3S7蛋白的三维结构，揭示了其与EIF3复合体其他亚基的相互作用界面，阐明其在翻译起始复合物组装中的关键作用。
2. **文献名称**：*EIF3S7 Overexpression Enhances Cap-dependent Translation in Cancer Cells*
**作者**：Zhang L et al.
**摘要**：研究利用重组人EIF3S7蛋白进行功能实验，发现其过表达促进乳腺癌细胞中依赖帽结构的蛋白质翻译，并与肿瘤增殖和耐药性相关。
3. **文献名称**：*Optimizing Recombinant Human EIF3S7 Expression in E. coli for Antibody Production*
**作者**：Wang Q et al.
**摘要**：报道一种高效重组表达和纯化人EIF3S7蛋白的方法，通过优化大肠杆菌表达系统的条件，获得高纯度蛋白用于单克隆抗体制备及后续诊断应用。
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**备注**：以上文献为虚构示例，实际研究中需通过PubMed或Web of Science等数据库检索具体文献。建议使用关键词“Recombinant human EIF3S7”或“EIF3S7 protein function”进行查阅。
Recombinant human EIF3S7 protein, also known as eukaryotic translation initiation factor 3 subunit H (EIF3H), is a critical component of the eukaryotic translation initiation complex eIF3. This multi-subunit complex plays a central role in initiating protein synthesis by recruiting ribosomes to mRNA templates. EIF3S7. encoded by the EIF3H gene, forms part of the conserved PCI (Proteasome-COP9-eIF3) domain-containing core of eIF3. interacting with other subunits to stabilize the complex and regulate its activity.
Studies link EIF3S7 to both canonical translation and specific regulatory pathways, including cell cycle progression and oncogenesis. Its overexpression has been observed in multiple cancers, where it potentially drives tumor growth by enhancing translation of oncogenic mRNAs. Recombinant EIF3S7 is typically produced in prokaryotic or eukaryotic expression systems, enabling functional studies on its structure, post-translational modifications, and interactions.
Current research focuses on its role in stress-adapted translation, viral infection responses, and as a potential therapeutic target. However, challenges remain in fully elucidating its isoform-specific functions due to alternative splicing variants. As a reagent, recombinant EIF3S7 supports investigations into translation dysregulation mechanisms and high-throughput screening for inhibitors in cancer contexts. Its structural complexity, including disordered regions, necessitates advanced expression strategies to maintain native conformation and activity.
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