| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EIF3S6 |
| Uniprot No | P60228 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-445aa |
| 氨基酸序列 | MAEYDLTTRIAHFLDRHLVFPLLEFLSVKEIYNEKELLQGKLDLLSDTNMVDFAMDVYKNLYSDDIPHALREKRTTVVAQLKQLQAETEPIVKMFEDPETTRQMQSTRDGRMLFDYLADKHGFRQEYLDTLYRYAKFQYECGNYSGAAEYLYFFRVLVPATDRNALSSLWGKLASEILMQNWDAAMEDLTRLKETIDNNSVSSPLQSLQQRTWLIHWSLFVFFNHPKGRDNIIDLFLYQPQYLNAIQTMCPHILRYLTTAVITNKDVRKRRQVLKDLVKVIQQESYTYKDPITEFVECLYVNFDFDGAQKKLRECESVLVNDFFLVACLEDFIENARLFIFETFCRIHQCISINMLADKLNMTPEEAERWIVNLIRNARLDAKIDSKLGHVVMGNNAVSPYQQVIEKTKSLSFRSQMLAMNIEKKLNQNSRSEAPNWATQDSGFY |
| 分子量 | 79.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人EIF3S6蛋白的3篇参考文献示例(注：部分文献标题与作者为示例性内容，请根据实际需求补充真实信息)：
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**1. 文献名称**: *The eukaryotic translation initiation factor eIF3e interacts with Smad4 to promote TGF-β-mediated EMT in breast cancer cells*
**作者**: Zhang, L. et al.
**摘要**: 本研究探讨了重组人EIF3S6(eIF3e)在乳腺癌细胞转化生长因子β(TGF-β)信号通路中的作用，发现其通过与Smad4蛋白相互作用，促进上皮间质转化(EMT)，增强癌细胞侵袭性。
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**2. 文献名称**: *Functional characterization of recombinant human EIF3S6 subunit in translation initiation complex assembly*
**作者**: Smith, J.A. & Kim, H.
**摘要**: 利用大肠杆菌表达系统制备重组人EIF3S6蛋白，研究其在真核翻译起始复合体(eIF3)中的结构功能，发现该亚基对复合体稳定性及核糖体结合能力起关键调控作用。
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**3. 文献名称**: *EIF3S6 as a potential biomarker in hepatocellular carcinoma: Overexpression and clinical implications*
**作者**: Wang, Y. et al.
**摘要**: 通过重组EIF3S6蛋白的体外实验，结合肝癌患者组织样本分析，揭示了该蛋白的异常高表达与肝癌进展及患者预后不良的相关性，提示其可作为潜在诊断标志物。
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如需具体文献，建议通过**PubMed**或**Web of Science**搜索关键词“recombinant EIF3S6”、“EIF3e function”或“eukaryotic initiation factor 3 subunit”获取最新研究。
Recombinant human EIF3S6 protein, also known as eukaryotic translation initiation factor 3 subunit F (EIF3F), is a critical component of the eukaryotic translation initiation factor 3 (eIF3) complex. This multifunctional complex plays a central role in initiating protein synthesis by facilitating the assembly of the 43S pre-initiation complex, recruiting ribosomal subunits, and modulating mRNA binding. The EIF3S6 subunit, encoded by the *EIF3F* gene located on chromosome 19q13.2. contains a conserved PCI (Proteasome-COP9-eIF3) domain involved in protein-protein interactions. Its structural and regulatory functions contribute to ribosome stability and selective translation of mRNAs, particularly under stress conditions.
As a recombinant protein, EIF3S6 is typically produced using expression systems like *E. coli* or mammalian cells, enabling studies of its biochemical properties and interactions. Researchers employ it to investigate eIF3's role in translation regulation, cell proliferation, and disease mechanisms. Dysregulation of EIF3S6 has been implicated in cancers, including hepatocellular carcinoma and leukemia, where altered expression correlates with tumor progression and poor prognosis. Recombinant EIF3S6 also aids in structural analyses, such as cryo-EM studies of the eIF3 complex, and screens for small-molecule inhibitors targeting translation initiation in cancer therapy. Its production in vitro allows functional dissection without interference from other cellular factors, making it a valuable tool in both basic and applied biomedical research.
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