| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EIF3S3 |
| Uniprot No | O15372 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-352aa |
| 氨基酸序列 | MASRKEGTGSTATSSSSTAGAAGKGKGKGGSGDSAVKQVQIDGLVVLKIIKHYQEEGQGTEVVQGVLLGLVVEDRLEITNCFPFPQHTEDDADFDEVQYQMEMMRSLRHVNIDHLHVGWYQSTYYGSFVTRALLDSQFSYQHAIEESVVLIYDPIKTAQGSLSLKAYRLTPKLMEVCKEKDFSPEALKKANITFEYMFEEVPIVIKNSHLINVLMWELEKKSAVADKHELLSLASSNHLGKNLQLLMDRVDEMSQDIVKYNTYMRNTSKQQQQKHQYQQRRQQENMQRQSRGEPPLPEEDLSKLFKPPQPPARMDSLLIAGQINTYCQNIKEFTAQNLGKLFMAQALQEYNN |
| 分子量 | 64.46 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人EIF3S3蛋白的3篇参考文献的简要总结(基于公开研究领域的内容示例)：
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1. **文献名称**: *Eukaryotic Translation Initiation Factor 3 Subunit S3 (eIF3S3) Interacts with Viral RNA to Promote Hepatitis C Virus Replication*
**作者**: Li, W., et al.
**摘要**: 本研究通过重组人EIF3S3蛋白的体外表达，揭示了其与丙型肝炎病毒RNA的相互作用机制，表明EIF3S3在病毒复制中的关键调控作用。
2. **文献名称**: *Functional Characterization of Eukaryotic Initiation Factor 3 Subunits in Mammalian Cells*
**作者**: Zhang, L., et al.
**摘要**: 利用重组EIF3S3蛋白进行功能缺失实验，发现该亚基对细胞周期调控和蛋白质翻译起始有显著影响，尤其在癌细胞中高表达。
3. **文献名称**: *Structural Insights into the Human Eukaryotic Translation Initiation Factor 3 Complex*
**作者**: Sun, C., et al.
**摘要**: 通过重组表达人EIF3复合体亚基(包括EIF3S3)，结合冷冻电镜技术解析其三维结构，阐明EIF3S3在复合体组装中的关键结合位点。
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**注**：以上文献标题及内容为领域相关示例，实际引用需根据具体文献数据库(如PubMed、Web of Science)检索确认。如需进一步精确检索，建议使用关键词 **"recombinant human EIF3S3"** 或 **"EIF3S3 protein function"** 结合筛选条件(如发表年份、实验方法)进行查询。
Recombinant human EIF3S3 protein, also known as eukaryotic translation initiation factor 3 subunit G (EIF3G), is a critical component of the eukaryotic translation initiation factor 3 (eIF3) complex. This multi-subunit complex plays a central role in initiating protein synthesis by recruiting ribosomes to mRNA, facilitating the assembly of translation machinery. EIF3S3 contributes to stabilizing the eIF3 structure and interacts with other initiation factors, ribosomes, and mRNA to ensure accurate translation initiation. Dysregulation of EIF3S3 has been implicated in various cancers, viral infections, and cellular stress responses, highlighting its importance in both normal physiology and disease.
The recombinant form of EIF3S3 is produced using biotechnological systems (e.g., *E. coli* or mammalian cell cultures*) to enable precise study of its function, interactions, and post-translational modifications. Researchers employ recombinant EIF3S3 to investigate mechanisms of translational control, screen for inhibitors targeting aberrant translation in cancer, or explore viral hijacking of host machinery. Its standardized production ensures reproducibility in biochemical assays, structural studies, and drug development.
Studies on recombinant EIF3S3 continue to uncover its role in mRNA specificity, ribosome remodeling, and signaling pathways, offering potential therapeutic avenues for diseases linked to translational dysregulation.
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