| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EHD4 |
| Uniprot No | Q9H223 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-541aa |
| 氨基酸序列 | MFSWMGRQAGGRERAGGADAVQTVTGGLRSLYLRKVLPLEEAYRFHEFHSPALEDADFENKPMILLVGQYSTGKTTFIRYLLEQDFPGMRIGPEPTTDSFIAVMYGETEGSTPGNALVVDPKKPFRKLSRFGNAFLNRFMCSQLPNQVLKSISVIDSPGILSGEKQRISRGYDFCQVLQWFAERVDRIILLFDAHKLDISDEFSEAIKAFRGQDDKIRVVLNKADQVDTQQLMRVYGALMWSLGKVINTPEVLRVYIGSFWAQPLQNTDNRRLFEAEAQDLFRDIQSLPQKAAVRKLNDLIKRARLAKVHAYIISYLKKEMPSVFGKENKKRELISRLPEIYIQLQREYQISAGDFPEVKAMQEQLENYDFTKFHSLKPKLIEAVDNMLSNKISPLMNLISQEETSTPTQLVQGGAFDGTTEGPFNQGYGEGAKEGADEEEWVVAKDKPVYDELFYTLSPINGKISGVNAKKEMVTSKLPNSVLGKIWKLADCDCDGMLDEEEFALAKHLIKIKLDGYELPSSLPPHLVPPSHRKSLPKAD |
| 分子量 | 85.25 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人EHD4蛋白的3篇参考文献摘要(示例基于近年研究方向，实际文献请通过学术数据库核实)：
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1. **文献名称**：*Structural Insights into the Membrane Remodeling Mechanism of Human EHD4 Protein*
**作者**：Smith A, et al.
**摘要**：通过X射线晶体学解析了重组人EHD4蛋白的ATP结合结构域结构，揭示了其与磷脂膜互作的关键位点，表明EHD4在调控内吞囊泡形成中的构象变化机制。
2. **文献名称**：*EHD4 Regulates Endocytic Recycling and Cell Migration through Interaction with Rab11-FIP3*
**作者**：Chen L, Wang Y.
**摘要**：研究报道重组人EHD4通过与Rab11效应蛋白FIP3相互作用，介导回收内体的膜运输，影响细胞迁移和肿瘤侵袭，为癌症治疗提供潜在靶点。
3. **文献名称**：*Functional Characterization of Recombinant EHD4 in Neurological Disorder Models*
**作者**：Kim S, et al.
**摘要**：利用HEK293细胞表达重组EHD4.发现其在神经元突触囊泡再循环中起关键作用，EHD4缺失导致小鼠模型突触传递异常，与神经退行性疾病相关。
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**说明**：以上内容为模拟示例，具体文献需通过PubMed、Google Scholar等平台用关键词“EHD4”、“recombinant EHD4 protein”或“EH domain-containing protein 4”检索获取。近年研究可能侧重EHD4在膜运输、疾病机制中的功能。
Recombinant human EHD4 protein is a engineered version of the Eps15 homology domain-containing protein 4 (EHD4), a member of the EHD protein family involved in membrane trafficking and endocytic recycling. EHD4. encoded by the EHD4 gene in humans, shares structural features with other EHD paralogs (EHD1-3), including a conserved N-terminal EH domain that binds proteins containing Asn-Pro-Phe (NPF) motifs, a central dynamin-like GTPase domain for membrane remodeling, and a C-terminal coiled-coil domain mediating oligomerization. Unlike its paralogs, EHD4 exhibits distinct tissue expression patterns and functional specializations, particularly in regulating trafficking from recycling endosomes to the plasma membrane and maintaining organelle morphology.
The recombinant protein is typically produced in heterologous systems like *E. coli* or mammalian cells using molecular cloning techniques, enabling controlled studies of its biochemical properties, interactome, and role in cellular processes. Researchers utilize purified EHD4 to investigate its ATPase activity, membrane-binding kinetics, and functional interplay with regulatory partners like Rab proteins and SNAREs. Dysregulation of EHD4 has been implicated in neurological disorders and cancers, making it a target for mechanistic studies in disease models. Its recombinant form also serves as an antigen for antibody development. Ongoing research aims to clarify isoform-specific functions and therapeutic potential in trafficking-related pathologies.
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