| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EFS |
| Uniprot No | O43281 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-561aa |
| 氨基酸序列 | MAIATSTQLA RALYDNTAES PQELSFRRGD VLRVLQREGA GGLDGWCLCS LHGQQGIVPA NRVKLLPAGP APKPSLSPAS PAQPGSPYPA PDHSNEDQEV YVVPPPARPC PTSGPPAGPC PPSPDLIYKI PRASGTQLAA PRDALEVYDV PPTALRVPSS GPYDCPASFS HPLTRVAPQP PGEDDAPYDV PLTPKPPAEL EPDLEWEGGR EPGPPIYAAP SNLKRASALL NLYEAPEELL ADGEGGGTDE GIYDVPLLGP EAPPSPEPPG ALASHDQDTL AQLLARSPPP PHRPRLPSAE SLSRRPLPAL PVPEAPSPSP VPSPAPGRKG SIQDRPLPPP PPRLPGYGGP KVEGDPEGRE MEDDPAGHHN EYEGIPMAEE YDYVHLKGMD KAQGSRPPDQ ACTGDPELPE RGMPAPQEAL SPGEPLVVST GDLQLLYFYA GQCQSHYSAL QAAVAALMSS TQANQPPRLF VPHSKRVVVA AHRLVFVGDT LGRLAASAPL RAQVRAAGTA LGQALRATVL AVKGAALGYP SSPAIQEMVQ CVTELAGQAL QFTTLLTSLA P |
| 分子量 | 58.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人EFS蛋白的示例参考文献(内容为虚构，仅供格式参考)：
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1. **文献名称**：*Expression and functional characterization of recombinant human EFS protein in cancer cell lines*
**作者**：Smith A, et al.
**摘要**：本研究成功在大肠杆菌中表达了重组人EFS蛋白，并验证其通过调控酪氨酸激酶信号通路抑制肿瘤细胞增殖的功能。结果表明，EFS在体外显著降低乳腺癌细胞迁移能力。
2. **文献名称**：*Structural insights into human EFS protein and its interaction with Fyn kinase*
**作者**：Lee J, et al.
**摘要**：通过X射线晶体学解析了重组人EFS蛋白的三维结构，揭示了其与Fyn激酶结合的关键结构域，为设计靶向EFS的抗癌药物提供理论依据。
3. **文献名称**：*Recombinant EFS enhances DNA damage response via ATM/ATR signaling*
**作者**：Garcia R, et al.
**摘要**：研究发现，重组人EFS蛋白可通过激活ATM/ATR通路促进DNA损伤修复，其缺失会导致细胞对电离辐射敏感性增加。
4. **文献名称**：*Development of a novel EFS-based biomarker assay for early cancer detection*
**作者**：Chen L, et al.
**摘要**：基于重组人EFS蛋白的免疫检测技术，开发了一种高灵敏度血清标志物检测方法，可用于多种实体瘤的早期筛查。
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注：上述文献为示例，实际引用需检索真实数据库(如PubMed、Web of Science)并核实原文。建议使用关键词“recombinant human EFS protein”“EFS function”“EFS cancer”等进一步查询。
Recombinant human EFS (Enhanced Fertility and Spermatogenesis) protein is a genetically engineered form of the endogenous EFS protein, primarily associated with reproductive biology and cancer research. The EFS gene, also known as **Epididymal Secretory Protein** or **Sperm-associated Antigen 16** (SPAG16), plays critical roles in spermatogenesis, sperm motility, and fertilization. Studies suggest its involvement in forming the sperm fibrous sheath and regulating microtubule dynamics during gamete development. Notably, EFS has dual implications: its dysregulation is linked to male infertility due to structural sperm defects, while it also exhibits tumor-suppressive properties. In cancers, EFS promoter hypermethylation leads to reduced expression, correlating with poor prognosis in breast, lung, and gastric cancers. Recombinant EFS protein is produced using expression systems (e.g., *E. coli* or mammalian cells) to study its molecular mechanisms, including interactions with histone acetyltransferases or cell cycle regulators. Its applications span *in vitro* fertilization research, cancer therapeutic development (e.g., demethylation therapies), and as a biochemical tool to explore epigenetic regulation. Ongoing research aims to clarify its tissue-specific roles and therapeutic potential.
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