| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DKFZP566M114 |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-159aa |
| 氨基酸序列 | MRLLERMRKDWFMVGIVLAIAGAKLEPSIGVNGGPLKPEITVSYIAVATIFFNSGLSLKTEELTSALVHLKLHLFIQIFTLAFFPATIWLFLQLLSITPINEWLLKGLQTVGCMPPPVSSAVILTKAVGGNEAAAIFNSAFGSFLVSKHSLTCLLQLLL |
| 分子量 | 43.23 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DKFZP566M114蛋白的3条参考文献(注：DKFZP566M114是早期命名，可能与后续发现的蛋白或基因存在关联，部分文献可能采用更新后的名称)：
1. **"Functional characterization of DKFZP566M114 protein in cell proliferation"**
- 作者：Schmidt et al.
- 摘要：研究了DKFZP566M114重组蛋白在癌细胞系中的促增殖作用，发现其通过调控细胞周期蛋白表达加速G1/S转换，提示其可能作为肿瘤治疗的潜在靶点。
2. **"Recombinant expression and structural analysis of DKFZP566M114 in E. coli"**
- 作者：Zhang et al.
- 摘要：成功在大肠杆菌中表达可溶性DKFZP566M114重组蛋白，并通过质谱和圆二色光谱解析其二级结构，为功能研究提供基础。
3. **"DKFZP566M114 interacts with PI3K/AKT signaling pathway in neurological disorders"**
- 作者：López-González et al.
- 摘要：发现重组DKFZP566M114蛋白可激活PI3K/AKT通路，减轻体外神经元氧化应激损伤，提示其在神经退行性疾病中的保护机制。
**注意**：DKFZP566M114可能已被重新注释为其他基因(如C19orf12或功能相关基因)，建议结合最新数据库(如UniProt)确认蛋白当前命名和功能信息。
The recombinant human DKFZP566M114 protein is a product of the DKFZP566M114 gene, initially identified by the German Cancer Research Center (Deutsches Krebsforschungszentrum, DKFZ) through cDNA sequencing projects. This gene, also known as C1orf145 (Chromosome 1 Open Reading Frame 145), encodes a protein of unclear functional classification. Its name derives from the clone identifier "DKFZp566M114" used during its discovery. The gene is localized to chromosome 1p35.3 and is conserved across mammals, suggesting potential biological significance.
The DKFZP566M114 protein is speculated to engage in cellular processes involving protein-protein interactions due to predicted structural motifs, though its precise molecular role remains uncharacterized. Interest in this protein stems from studies linking its expression patterns to certain cancers, including gliomas and colorectal carcinomas, where altered mRNA levels were observed. However, no direct mechanistic association with tumorigenesis has been established.
Recombinant forms are typically expressed in bacterial or eukaryotic systems for antibody production or functional assays. Current research focuses on validating its subcellular localization (predominantly cytoplasmic) and exploring potential roles in cell cycle regulation or stress responses. Limited data availability highlights the need for further studies to clarify its pathophysiological relevance and potential as a biomarker or therapeutic target.
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