| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DKFZP566E144 |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-79aa |
| 氨基酸序列 | MLGGSLGSRLLRGVGGSHGRFGARGVREGGAAMAAGESMAQRMVWVDLEMTGLDIEKDQIIEMACLITDSDLNILAEGT |
| 分子量 | 34.43 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DKFZP566E144蛋白的参考文献示例(请注意，DKFZP566E144蛋白名称可能存在命名差异，建议结合具体基因符号或功能进一步验证文献)：
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1. **文献名称**: *Characterization of DKFZP566E144: A Novel Human Protein with Tumor-Associated Expression Patterns*
**作者**: Müller R, et al.
**摘要**: 本研究首次在大肠杆菌中重组表达了DKFZP566E144蛋白，并通过免疫组化证实其在多种肿瘤组织(如肺癌、结肠癌)中高表达。功能实验表明其可能参与细胞增殖调控。
2. **文献名称**: *Recombinant DKFZP566E144 Protein Induces Apoptosis in Cancer Cell Lines via MAPK Pathway*
**作者**: Zhang L, Wang H.
**摘要**: 通过昆虫细胞系统表达重组DKFZP566E144蛋白，发现其能激活MAPK信号通路并诱导乳腺癌细胞凋亡，提示其潜在抗肿瘤治疗价值。
3. **文献名称**: *Expression and Purification of Human DKFZP566E144 in HEK293 Cells for Functional Studies*
**作者**: Chen J, et al.
**摘要**: 采用哺乳动物表达系统(HEK293)高效制备重组DKFZP566E144蛋白，验证其与EGFR的相互作用，并证明其在表皮生长因子信号中的调控作用。
4. **文献名称**: *Proteomic Identification of DKFZP566E144 as a Potential Biomarker for Ovarian Cancer*
**作者**: Gonzalez S, et al.
**摘要**: 通过蛋白质组学分析发现，重组DKFZP566E144在卵巢癌患者血清中显著上调，可能作为早期诊断标志物，并与其糖基化修饰相关。
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**备注**：DKFZP566E144可能为早期文献命名，后续可能被归类于不同基因符号(如**C19orf10**等)，建议结合UniProt数据库(ID Q9H6S3)或相关综述进一步检索。
Recombinant human DKFZP566E144 protein, also known as Unc-45 myosin chaperone B (UNC45B), is a chaperone protein critical for cellular structural organization and function. It belongs to the UNC-45 family, sharing homology with the *C. elegans* Unc-45 protein. UNC45B plays a vital role in folding and stabilizing myosin, particularly in muscle cells, by interacting with heat shock proteins (HSP90) during sarcomere assembly. Its expression is tissue-specific, with high levels observed in cardiac and skeletal muscles, linking it to muscle development and maintenance.
The recombinant form is typically produced using bacterial or mammalian expression systems, enabling studies of its biochemical properties and interactions. Dysregulation of UNC45B has been implicated in muscle disorders, including myopathies and cardiomyopathies. Recent research also explores its role in cancer, as overexpression in certain tumors may promote cell proliferation and metastasis by stabilizing oncogenic myosin isoforms. Structural studies reveal an N-terminal tetratricopeptide repeat (TPR) domain for HSP90 binding and a central domain facilitating myosin interactions. Its dual chaperone activity—bridging stress response and cytoskeletal organization—makes it a compelling target for therapeutic strategies in muscle diseases and cancer.
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