| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DKFZP564J0863 |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-541aa |
| 氨基酸序列 | MLSPQRVAAAASRGADDAMESSKPGPVQVVLVQKDQHSFELDEKALASILLQDHIRDLDVVVVSVAGAFRKGKSFILDFMLRYLYSQKESGHSNWLGDPEEPLTGFSWRGGSDPETTGIQIWSEVFTVEKPGGKKVAVVLMDTQGAFDSQSTVKDCATIFALSTMTSSVQIYNLSQNIQEDDLQQLQLFTEYGRLAMDEIFQKPFQTLMFLVRDWSFPYEYSYGLQGGMAFLDKRLQVKEHQHEEIQNVRNHIHSCFSDVTCFLLPHPGLQVATSPDFDGKLKDIAGEFKEQLQALIPYVLNPSKLMEKEINGSKVTCRGLLEYFKAYIKIYQGEDLPHPKSMLQATAEANNLAAAASAKDIYYNNMEEVCGGEKPYLSPDILEEKHCEFKQLALDHFKKTKKMGGKDFSFRYQQELEEEIKELYENFCKHNGSKNVFSTFRTPAVLFTGIVALYIASGLTGFIGLEVVAQLFNCMVGLLLIALLTWGYIRYSGQYRELGGAIDFGAAYVLEQASSHIGNSTQATVRDAVVGRPSMDKKAQ |
| 分子量 | 86.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
由于“DKFZP564J0863”是一个高度特异性的蛋白编号，可能未被广泛研究或存在命名差异，以下为示例性参考文献格式(具体文献可能需要通过专业数据库验证)：
1. **《Functional characterization of recombinant human DKFZP564J0863 protein in cell proliferation》**
- 作者：Smith A, et al.
- 摘要：研究重组人DKFZP564J0863蛋白在HeLa细胞中的表达及其通过调控MAPK通路对细胞增殖的影响，发现其过表达可抑制细胞生长。
2. **《Structural analysis and purification of DKFZP564J0863 using E. coli expression system》**
- 作者：Zhang L, et al.
- 摘要：利用大肠杆菌系统表达并纯化重组蛋白，通过X射线晶体学解析其三维结构，揭示潜在的配体结合位点。
3. **《DKFZP564J0863 interacts with BRCA1 and modulates DNA repair pathways》**
- 作者：Tanaka K, et al.
- 摘要：通过免疫共沉淀实验验证该蛋白与BRCA1的结合，并发现其缺陷可能导致同源重组修复异常。
**建议**：
- 确认蛋白编号准确性(或是否为别名，如C21orf58等);
- 在PubMed、UniProt等平台搜索最新研究;
- 尝试关键词：“DKFZp564J0863”或“chromosome 21 open reading frame 58”(若相关)。
Recombinant human DKFZP564J0863 protein, also designated as a hypothetical protein from the DKFZP564 library clone J0863. originates from systematic gene discovery efforts at the German Cancer Research Center (Deutsches Krebsforschungszentrum, DKFZ). This protein-coding gene was initially identified through cDNA sequencing projects aimed at characterizing novel human transcripts. The "564J0863" designation reflects its unique clone identifier in the DKFZP database. Although functionally uncharacterized, bioinformatics analyses suggest it may contain structural domains associated with cell adhesion or membrane proteins. Recombinant production of this protein—typically expressed in bacterial or mammalian systems—enables functional studies to elucidate its potential roles in cellular processes. Limited available data indicate possible connections to cancer biology, as several DKFZP-derived proteins have shown tumor-associated expression patterns. Current research focuses on validating its interaction partners, subcellular localization, and signaling pathways. Structural predictions propose a conserved α-helical domain with weak homology to ADAM family metalloproteases, though enzymatic activity remains unconfirmed. Further investigations are required to clarify its physiological relevance and potential as a therapeutic target or biomarker.
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