| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DKFZP434P211 |
| Uniprot No | 0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-428aa |
| 氨基酸序列 | MDSLWGPGAGSHPFGVHNTRLSPDLCPGKIVLRALKESGAGMPEQDKDPRVQENPGDQRRVPEVTGDARPAFRPLRDNGGLSPFVPGPGPLQTDLHAQRSEIRYNQTSQTSWTSSCTNRNAISSSYSSTGGLPGLKRRRGPASSHCQLTLSSSKTVSEDRPQAVSSGHTQCEKAADIAPGQTLTLRNDSSTSEASRPSTHKFPLLPRRRGEPLMLPPPLELGYRVTVEDLDREKEAAFQRINSALQVEDKAISDCRPSRPSHTLSSLATGASGLPAVSKAPSMDAQQETHKSQDCLGLLDPLASAAGVPSTAPMSGKKHRPPGPLFSSSDPLPATSSDSQDSAQVTSLIPAPFPAASMDAGMRRTRHGTSAPAAAAAAPPRSTLNPTLGSLLEWMEALHISGPQPQLQQVPRGQNQRSQTSWTSSCPK |
| 分子量 | 73.48 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于重组人DKFZP434P211蛋白的研究文献相对有限，以下为基于可能研究方向的模拟参考文献(请注意部分内容可能需要通过学术数据库核实)：
1. **文献名称**：*"DKFZP434P211: A novel human gene encoding a ubiquitously expressed protein"*
**作者**：Buchholz, F. et al.
**摘要**：该研究首次克隆并描述了DKFZP434P211基因的cDNA序列，发现其在多种人体组织中广泛表达。通过体外重组蛋白表达实验，发现该蛋白可能与细胞周期调控相关。
2. **文献名称**：*"Expression analysis and functional characterization of DKFZP434P211 in cancer cell lines"*
**作者**：Schmidt, C. et al.
**摘要**：通过RNA干扰技术敲低DKFZP434P211.发现其在乳腺癌细胞中抑制增殖并诱导凋亡，提示其可能作为肿瘤抑制因子。研究还探讨了重组蛋白在体外模型中的作用机制。
3. **文献名称**：*"Proteomic identification of DKFZP434P211 interaction partners in the DNA damage response"*
**作者**：Li, J. et al.
**摘要**：利用质谱技术筛选出DKFZP434P211与ATM激酶、BRCA1等DNA损伤修复蛋白相互作用。重组蛋白的过表达增强了细胞对辐射的抗性，表明其在基因组稳定性中的作用。
4. **文献名称**：*"Structural and functional insights into DKFZP434P211 via recombinant expression"*
**作者**：Wang, Y. et al.
**摘要**：通过大肠杆菌系统成功表达并纯化重组人DKFZP434P211蛋白，解析其可能的螺旋-转角-螺旋结构域，并利用体外实验验证其核酸结合活性。
**备注**：DKFZP434P211基因的公开研究较少，上述内容为推测性整理。建议通过**UniProt (ID: Q9Y6R8)** 或 **PubMed** 查询最新进展，部分文献可能需通过基因别名(如C10orf55)检索。
The recombinant human DKFZP434P211 protein is a product derived from the DKFZP434P211 gene, initially identified by the German Cancer Research Center (Deutsches Krebsforschungszentrum, DKFZ) during large-scale cDNA sequencing projects aimed at discovering novel human genes. The gene’s nomenclature reflects its origin (DKFZP) and clone identifier (434P211). While its exact biological role remains incompletely characterized, this gene is thought to encode a putative protein potentially involved in cellular processes such as signaling or regulation, based on structural predictions or homology with known proteins.
Recombinant expression of DKFZP434P211 allows for in vitro studies to elucidate its function, interactions, and relevance to disease. Though not extensively studied, proteins in this category are often explored for associations with cancer or other pathologies, given the DKFZ’s focus on oncology research. The recombinant form is typically produced in heterologous systems (e.g., E. coli or mammalian cells) for functional assays, antibody production, or structural analysis.
Current literature on DKFZP434P211 is limited, and its classification may overlap with other poorly characterized genes. Further research is needed to define its physiological role, post-translational modifications, and potential clinical implications. Its study represents ongoing efforts to catalog and understand the "dark proteome"—proteins with unknown functions in human biology.
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