| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DIMT1L |
| Uniprot No | Q9UNQ2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-313aa |
| 氨基酸序列 | MPKVKSGAIGRRRGRQEQRRELKSAGGLMFNTGIGQHILKNPLIINSIIDKAALRPTDVVLEVGPGTGNMTVKLLEKAKKVVACELDPRLVAELHKRVQGTPVASKLQVLVGDVLKTDLPFFDTCVANLPYQISSPFVFKLLLHRPFFRCAILMFQREFALRLVAKPGDKLYCRLSINTQLLARVDHLMKVGKNNFRPPPKVESSVVRIEPKNPPPPINFQEWDGLVRITFVRKNKTLSAAFKSSAVQQLLEKNYRIHCSVHNIIIPEDFSIADKIQQILTSTGFSDKRARSMDIDDFIRLLHGFNAEGIHFS |
| 分子量 | 51.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DIMT1L蛋白的参考文献及其简要摘要：
1. **文献名称**: *Structural and functional characterization of human DIMT1L in ribosome assembly*
**作者**: Zhang Y, et al.
**摘要**: 本研究解析了人源DIMT1L蛋白的晶体结构，揭示其作为甲基转移酶在核糖体RNA加工中的作用。通过重组表达纯化DIMT1L，验证其对28S rRNA特定腺苷残基的甲基化功能，并证明其缺陷影响核糖体亚基组装。
2. **文献名称**: *DIMT1L-mediated m6A modification regulates hematopoietic stem cell differentiation*
**作者**: Wang L, et al.
**摘要**: 利用重组人DIMT1L蛋白进行体外酶活实验，发现其催化m6A RNA修饰的机制，并通过敲低实验证明该修饰在造血干细胞分化中的调控作用，提示其与血液系统疾病的潜在关联。
3. **文献名称**: *Recombinant DIMT1L expression in E. coli and enzymatic activity screening*
**作者**: Müller S, et al.
**摘要**: 报道了在大肠杆菌中高效表达可溶性重组DIMT1L蛋白的优化策略，通过体外甲基转移酶活性检测，验证了其底物特异性及金属离子依赖特性，为大规模制备功能性DIMT1L提供方案。
4. **文献名称**: *DIMT1L interacts with viral RNA to modulate host antiviral response*
**作者**: Chen X, et al.
**摘要**: 通过重组人DIMT1L蛋白与病毒RNA的互作实验，发现其通过甲基化修饰抑制病毒复制，并调控干扰素信号通路。该研究揭示了DIMT1L在先天免疫中的新功能。
注：以上文献为示例性内容，实际需根据具体研究在学术数据库中检索验证。
Recombinant human DIMT1L (DIM1 dimethyladenosine transferase 1-like) protein is a genetically engineered version of the endogenous enzyme encoded by the *DIMT1L* gene. DIMT1L belongs to the methyltransferase superfamily and is primarily known for its role in RNA modification, particularly in ribosomal RNA (rRNA) processing. It catalyzes the dimethylation of two adjacent adenosines in the loop of 18S rRNA, a critical step in ribosome biogenesis. This post-transcriptional modification ensures the structural integrity and functional maturation of the small ribosomal subunit, thereby influencing translational fidelity and cellular protein synthesis.
Studies link DIMT1L to developmental processes, stem cell maintenance, and tumorigenesis. Its dysregulation has been implicated in cancers, neurological disorders, and developmental defects, underscoring its importance in cellular homeostasis. Recombinant DIMT1L protein, typically produced in *E. coli* or mammalian expression systems, retains enzymatic activity and is utilized to study methylation mechanisms, ribosome assembly, and disease pathways. It also serves as a tool for screening inhibitors or modulators targeting rRNA-modifying enzymes. Structural analyses reveal conserved SAM-binding motifs and catalytic domains, enabling comparative studies with homologs like yeast Dim1. Ongoing research aims to clarify its regulatory networks and therapeutic potential in diseases linked to translational dysregulation.
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