| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DHX58 |
| Uniprot No | Q96C10 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-678aa |
| 氨基酸序列 | MELRSYQWEVIMPALEGKNIIIWLPTGAGKTRAAAYVAKRHLETVDGAKVVVLVNRVHLVTQHGEEFRRMLDGRWTVTTLSGDMGPRAGFGHLARCHDLLICTAELLQMALTSPEEEEHVELTVFSLIVVDECHHTHKDTVYNVIMSQYLELKLQRAQPLPQVLGLTASPGTGGASKLDGAINHVLQLCANLDTWCIMSPQNCCPQLQEHSQQPCKQYNLCHRRSQDPFGDLLKKLMDQIHDHLEMPELSRKFGTQMYEQQVVKLSEAAALAGLQEQRVYALHLRRYNDALLIHDTVRAVDALAALQDFYHREHVTKTQILCAERRLLALFDDRKNELAHLATHGPENPKLEMLEKILQRQFSSSNSPRGIIFTRTRQSAHSLLLWLQQQQGLQTVDIRAQLLIGAGNSSQSTHMTQRDQQEVIQKFQDGTLNLLVATSVAEEGLDIPHCNVVVRYGLLTNEISMVQARGRARADQSVYAFVATEGSRELKRELINEALETLMEQAVAAVQKMDQAEYQAKIRDLQQAALTKRAAQAAQRENQRQQFPVEHVQLLCINCMVAVGHGSDLRKVEGTHHVNVNPNFSNYYNVSRDPVVINKVFKDWKPGGVISCRNCGEVWGLQMIYKSVKLPVLKVRSMLLETPQGRIQAKKWSRVPFSVPDFDFLQHCAENLSDLSLD |
| 分子量 | 103 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DHX58蛋白的3篇参考文献示例(文献信息为模拟示例，非真实数据)：
**1. 文献名称**：*DHX58 in antiviral immunity: Dual regulation of RIG-I signaling*
**作者**：Yoneyama, M., et al.
**摘要**：研究解析了重组人DHX38蛋白在RNA病毒识别中的作用，发现其通过调控RIG-I通路激活I型干扰素，同时抑制过度炎症反应，平衡宿主抗病毒免疫应答。
**2. 文献名称**：*Structural insights into the RNA recognition mechanism of DHX58*
**作者**：Chen, Z., et al.
**摘要**：通过晶体结构分析重组人DHX58蛋白的CTD结构域，揭示其RNA结合模式及与RIG-I协同激活下游信号通路的分子机制。
**3. 文献名称**：*Recombinant DHX58 expression and its role in autoimmune diseases*
**作者**：Saito, T., et al.
**摘要**：利用HEK293系统表达重组DHX58.验证其在细胞中负向调控自身免疫反应的功能，提示其作为潜在治疗靶点的可能性。
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如需真实文献，建议通过PubMed或Google Scholar搜索关键词 "recombinant DHX38" 或 "LGP2 protein"，筛选近年发表的实验性研究(DHX58别称LGP2.与先天免疫相关)。
Recombinant human DHX58 protein, also known as LGP2 (Laboratory of Genetics and Physiology 2), is a key component of the innate immune system, primarily involved in antiviral responses. As a member of the RIG-I-like receptor (RLR) family, DHX58 acts as a cytoplasmic RNA helicase that detects viral nucleic acids, particularly double-stranded RNA (dsRNA), to initiate immune signaling. Unlike its homologs RIG-I and MDA5. DHX58 lacks the caspase activation and recruitment domains (CARDs) required for direct downstream signaling, suggesting its regulatory role in modulating RLR-mediated antiviral pathways. It fine-tunes immune responses by either enhancing or suppressing signaling via interactions with RIG-I and MDA5. depending on viral context.
Structurally, DHX58 contains a DExD/H-box helicase domain for RNA binding and ATP hydrolysis, along with a C-terminal regulatory domain. Its recombinant form is widely produced in bacterial or mammalian expression systems for functional studies. Research focuses on its dual role in viral infection—promoting interferon production during early infection while limiting excessive inflammation to prevent host damage. Dysregulation of DHX58 is linked to autoimmune diseases and viral immune evasion mechanisms. Recombinant DHX58 serves as a vital tool for elucidating host-pathogen interactions, screening antiviral compounds, and developing immunotherapies targeting RLR signaling pathways.
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