| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DHCR24 |
| Uniprot No | Q15392 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-516aa |
| 氨基酸序列 | MEPAVSLAVCALLFLLWVRLKGLEFVLIHQRWVFVCLFLLPLSLIFDIYYYVRAWVVFKLSSAPRLHEQRVRDIQKQVREWKEQGSKTFMCTGRPGWLTVSLRVGKYKKTHKNIMINLMDILEVDTKKQIVRVEPLVTMGQVTALLTSIGWTLPVLPELDDLTVGGLIMGTGIESSSHKYGLFQHICTAYELVLADGSFVRCTPSENSDLFYAVPWSCGTLGFLVAAEIRIIPAKKYVKLRFEPVRGLEAICAKFTHESQRQENHFVEGLLYSLDEAVIMTGVMTDEAEPSKLNSIGNYYKPWFFKHVENYLKTNREGLEYIPLRHYYHRHTRSIFWELQDIIPFGNNPIFRYLFGWMVPPKISLLKLTQGETLRKLYEQHHVVQDMLVPMKCLQQALHTFQNDIHVYPIWLCPFILPSQPGLVHPKGNEAELYIDIGAYGEPRVKHFEARSCMRQLEKFVRSVHGFQMLYADCYMNREEFWEMFDGSLYHKLREKLGCQDAFPEVYDKICKAARH |
| 分子量 | 86.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇关于重组人DHCR24蛋白的模拟参考文献示例(供参考)：
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1. **文献名称**：**"Expression and Enzymatic Activity of Recombinant Human DHCR24 in a Baculovirus System"**
**作者**：Chen L, Wang Y, et al.
**摘要**：本研究成功利用杆状病毒表达系统表达并纯化了具有生物活性的重组人DHCR24蛋白，证实其可通过催化脱氢胆固醇还原反应参与胆固醇合成途径，为体外研究该酶的动力学特性提供了工具。
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2. **文献名称**：**"Structural Insights into Human DHCR24: A Cryo-EM Study of the Recombinant Enzyme"**
**作者**：Tanaka K, Schmidt R.
**摘要**：通过冷冻电镜技术解析了重组人DHCR24蛋白的三维结构，揭示了其底物结合口袋及活性位点的关键氨基酸残基，为开发靶向胆固醇代谢疾病的药物奠定结构基础。
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3. **文献名称**：**"Role of Recombinant DHCR24 in Amyloid-β-Induced Neurotoxicity: Implications for Alzheimer's Disease"**
**作者**：Zhang H, Liu X, et al.
**摘要**：研究发现重组人DHCR24蛋白在神经元细胞中过表达可减少β-淀粉样蛋白毒性，机制与其调节细胞内胆固醇稳态并抑制氧化应激有关，提示DHCR24在阿尔茨海默病中的潜在治疗价值。
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*注：以上文献为基于领域知识的模拟示例，实际研究请通过学术数据库(如PubMed、Web of Science)检索最新文献。*
Human DHCR24 (24-dehydrocholesterol reductase) is a key enzyme encoded by the *DHCR24* gene located on chromosome 1 (1p33-p31.1). It catalyzes the final step in cholesterol biosynthesis, reducing the Δ24 double bond of desmosterol to produce cholesterol. Beyond lipid metabolism, DHCR24 is implicated in oxidative stress response, apoptosis regulation, and neuroprotection. Its expression is associated with the endoplasmic reticulum and regulated by sterol levels via the SREBP pathway.
Recombinant human DHCR24 protein is generated using expression systems like *E. coli*, yeast, or mammalian cells (e.g., HEK293), often fused with affinity tags (e.g., His-tag) for purification. This engineered protein retains enzymatic activity, enabling functional studies on cholesterol synthesis disorders (e.g., Smith-Lemli-Opitz syndrome) and diseases linked to DHCR24 dysfunction, including Alzheimer’s disease, atherosclerosis, and cancers. Structural analyses reveal conserved domains critical for catalytic activity, such as flavin-binding motifs.
Research applications include enzyme kinetics, drug screening for cholesterol-lowering therapies, and exploring DHCR24’s role in cellular stress resistance. Its dual function as a cholesterol synthase and redox sensor highlights its therapeutic potential in metabolic and neurodegenerative diseases.
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