| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DGKI |
| Uniprot No | O75912 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1057aa |
| 氨基酸序列 | MDAAGRGCHLLPLPAARGPARAPAAAAAAAASPPGPCSGAACAPSAAAGAGAMNPSSSAGEEKGATGGSSSSGSGAGSCCLGAEGGADPRGAGSAAAAGAAALDEPAAAGQKEKDEALEEKLRNLTFRKQVSYRKAISRAGLQHLAPAHPLSLPVANGPAKEPRATLDWSENAVNGEHLWLETNVSGDLCYLGEENCQVRFAKSALRRKCAVCKIVVHTACIEQLEKINFRCKPTFREGGSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKEIVAISCSWCKQAFHNKVTCFMLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQNSLKASNRKKKRTSFKRKASKRGMEQENKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALELYRKVPNLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVEDGTVVQLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRNKMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHHDFEPQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQANMVQKSKRRTSMPLLNDPQSVPDRLRIRVNKISLQDYEGFHYDKEKLREASIPLGILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDHETSFPRALSAQRLSPRWCFLDATSADRFYRIDRSQEHLHFVMEISQDEIFILDPDMVVSQPAGTPPGMPDLVVEQASGISDWWNPALRKRMLSDSGLGMIAPYYEDSDLKDLSHSRVLQSPVSSEDHAILQAVIAGDLMKLIESYKNGGSLLIQGPDHCSLLHYAAKTGNGEIVKYILDHGPSELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRKTDSKGKTPQERAQQAGDPDLAAYLESRQNYKVIGHEDLETAV |
| 分子量 | 116.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DGKI蛋白的模拟参考文献示例(虚构内容，仅供参考)：
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1. **文献名称**：*Expression and purification of recombinant human DGKI in a prokaryotic system for functional studies*
**作者**：Chen L, Wang X
**摘要**：本研究成功在大肠杆菌中表达并纯化了带有His标签的重组人DGKI蛋白，利用亲和层析和凝胶过滤技术获得高纯度蛋白。体外酶活实验显示DGKI可特异性催化二酰甘油(DAG)生成磷脂酸(PA)，为其在脂质信号通路中的功能研究奠定基础。
2. **文献名称**：*DGKI regulates cell migration through interaction with PKCδ in cancer cells*
**作者**：Kim S, Patel R
**摘要**：通过CRISPR-Cas9敲低和过表达实验，发现DGKI通过调控DAG-PKCδ信号轴影响肿瘤细胞迁移。重组DGKI蛋白的添加可逆转DAG代谢失衡，提示其作为潜在治疗靶点的可能性。
3. **文献名称**：*Structural insights into the catalytic mechanism of human DGKI by cryo-EM*
**作者**：Garcia M, Tanaka K
**摘要**：首次解析了重组人DGKI蛋白的冷冻电镜结构，揭示其ATP结合域和底物识别位点的关键氨基酸残基。分子动力学模拟表明，DGKI构象变化通过调控DAG/PA平衡影响下游通路。
4. **文献名称**：*Dysregulation of DGKI in glioblastoma and its role in tumor progression*
**作者**：Zhang Y, Liu T
**摘要**：分析胶质瘤临床样本发现DGKI表达显著上调。体外实验证实，重组DGKI蛋白可增强肿瘤细胞侵袭能力，而抑制剂处理可抑制体内肿瘤生长，表明其作为预后标志物的潜力。
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注：以上内容为模拟生成，实际文献需通过学术数据库检索。如需真实参考文献，建议在PubMed或Web of Science中搜索关键词“human DGKI”或“recombinant DGKI kinase”。
Recombinant human DGKI (diacylglycerol kinase iota) protein is a key enzyme involved in lipid metabolism and cellular signaling. DGKI belongs to the diacylglycerol kinase (DGK) family, which phosphorylates diacylglycerol (DAG) to generate phosphatidic acid (PA), thereby regulating the balance of these two lipid secondary messengers. DAG and PA play critical roles in signaling pathways, including protein kinase C (PKC) activation, mTOR signaling, and membrane trafficking. As a member of the type IV DGK subfamily, DGKI is distinguished by its unique structural features, such as a pleckstrin homology (PH) domain, a sterile alpha motif (SAM), and a C-terminal catalytic domain, which influence its subcellular localization and functional interactions.
DGKI is predominantly expressed in the brain, testis, and immune cells, suggesting roles in neural function, reproduction, and immune responses. Studies link it to processes like cell proliferation, apoptosis, and cytoskeletal reorganization. Dysregulation of DGKI has been implicated in neurological disorders, cancer, and metabolic syndromes, making it a potential therapeutic target. Recombinant DGKI protein, produced via heterologous expression systems (e.g., E. coli or mammalian cells), is essential for in vitro studies to dissect its enzymatic activity, structure-function relationships, and interactions with regulatory molecules. It also serves as a tool for drug discovery, particularly in developing inhibitors to modulate lipid signaling pathways implicated in disease.
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