| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DGCR2 |
| Uniprot No | P98153 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-550aa |
| 氨基酸序列 | MVPKADSGAFLLLFLLVLTVTEPLRPELRCNPGQFACRSGTIQCIPLPWQCDGWATCEDESDEANCPEVTGEVRPHHGKEAVDPRQGRARGGDPSHFHAVNVAQPVRFSSFLGKCPTGWHHYEGTASCYRVYLSGENYWDAAQTCQRLNGSLATFSTDQELRFVLAQEWDQPERSFGWKDQRKLWVGYQYVITGRNRSLEGRWEVAFKGSSEVFLPPDPIFASAMSENDNVFCAQLQCFHFPTLRHHDLHSWHAESCYEKSSFLCKRSQTCVDIKDNVVDEGFYFTPKGDDPCLSCTCHGGEPEMCVAALCERPQGCQQYRKDPKECCKFMCLDPDGNSLFDSMASGMRLVVSCISSFLILSLLLFMVHRLRQRRRERIESLIGANLHHFNLGRRIPGFDYGPDGFGTGLTPLHLSDDGEGGTFHFHDPPPPYTAYKYPDIGQPDDPPPPYEASIHPDSVFYDPADDDAFEPVEVSLPAPGDGGSEGALLRRLEQPLPTAGASLADLEDSADSSSALLVPPDPAQSGSTPAAEALPGGGRHSRSSLNTVV |
| 分子量 | 87.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DGCR2蛋白的3篇参考文献示例，涵盖其功能、表达及与疾病的关系：
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1. **文献名称**：**"DGCR2 functions as a non-canonical protease in protein quality control"**
**作者**：Lindsay, E.A., et al.
**摘要**：该研究报道了人DGCR2蛋白的重组表达及功能分析。通过在大肠杆菌中表达His标签的DGCR2.发现其具有ATP依赖的蛋白酶活性，可能参与错误折叠蛋白的降解。实验进一步验证了DGCR2在DiGeorge综合征相关细胞模型中的功能缺失表型。
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2. **文献名称**：**"Expression and structural characterization of recombinant human DGCR2 extracellular domain"**
**作者**：Shiohama, A., et al.
**摘要**：作者利用昆虫细胞系统表达了重组人DGCR2的胞外结构域，并通过X射线晶体学解析其三维结构。结果表明，该区域含有保守的亮氨酸重复序列，提示其在细胞粘附或受体相互作用中的潜在作用。
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3. **文献名称**：**"DGCR2 modulates neurotransmitter receptor trafficking via ubiquitination pathways"**
**作者**：Maynard, T.M., et al.
**摘要**：研究通过体外重组DGCR2蛋白实验，揭示了其在泛素化通路中的调节作用。DGCR2与E3泛素连接酶相互作用，影响神经元膜受体的稳定性，为精神分裂症的发病机制提供了分子层面的解释。
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**备注**：以上文献信息为模拟示例，实际文献需通过PubMed、Web of Science等平台检索关键词“recombinant human DGCR2”“DGCR2 expression”等获取。
Recombinant human DGCR2 (DiGeorge syndrome critical region gene 2) protein is derived from the DGCR2 gene located on chromosome 22q11.2. a region associated with DiGeorge syndrome, a congenital disorder characterized by cardiac defects, immune deficiencies, and neurodevelopmental abnormalities. The DGCR2 gene lies within the commonly deleted interval in 22q11.2 deletion syndrome and is considered a candidate gene contributing to the disorder’s pathophysiology. The encoded DGCR2 protein is a transmembrane glycoprotein containing multiple leucine-rich repeats, suggesting roles in protein-protein interactions, cellular adhesion, or signaling pathways. Structurally, it comprises an extracellular domain, a transmembrane region, and a short cytoplasmic tail, though its exact biological functions remain partially elusive.
Studies indicate DGCR2 may influence neural and cardiovascular development, potentially interacting with extracellular matrix components or modulating cell surface receptor activities. Dysregulation of DGCR2 has been implicated in psychiatric conditions like schizophrenia and bipolar disorder, possibly linked to its neurodevelopmental roles. Recombinant DGCR2 protein, produced via heterologous expression systems (e.g., mammalian or insect cells), enables functional studies, including ligand-receptor binding assays, structural analysis, and exploration of its involvement in cellular processes like apoptosis or differentiation. It also serves as a tool for investigating pathogenic mechanisms of 22q11.2 deletion-related disorders and screening therapeutic candidates. Research on recombinant DGCR2 continues to clarify its molecular interactions and disease relevance, offering insights into targeted interventions for associated syndromes.
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