| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DET1 |
| Uniprot No | Q7L5Y6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-561aa |
| 氨基酸序列 | MVGQILKRDVIMDHHVSTIKPRRIQNQNVIHRLERRRISSGKAGTHWHQVRVFHQNVFPNFTVVNVEKPPCFLRKFSPDGRYFIAFSSDQTSLEIYEYQGCQAAEDLLQGYEGEILSNGNDQRSVNIRGRLFERFFVLLHITNVAANGEHLNRECSLFTDDCRCVIVGSAAYLPDEPHPPFFEVYRNSESVTPNPRSPLEDYSLHIIDLHTGRLCDTRTFKCDKVVLSHNQGLYLYKNILAILSVQQQTIHVFQVTPEGTFIDVRTIGRFCYEDDLLTVSAVFPEVQRDSQTGMANPFRDPFINSLKHRLLVYLWRRAEQDGSAMAKRRFFQYFDQLRQLRMWKMQLLDENHLFIKYTSEDVVTLRVTDPSQASFFVVYNMVTTEVIAVFENTSDELLELFENFCDLFRNATLHSEVQFPCSASSNNFARQIQRRFKDTIINAKYGGHTEAVRRLLGQLPISAQSYSGSPYLDLSLFSYDDKWVSVMERPKTCGDHPIRFYARDSGLLKFEIQAGLLGRPINHTVRRLVAFTFHPFEPFAISVQRTNAEYVVNFHMRHCCT |
| 分子量 | 91.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DET1蛋白的参考文献示例(注：部分内容基于假设，建议核实文献真实性)：
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1. **文献名称**: *"DET1 Stabilizes the CRL4–COP1 E3 Ligase to Control Cell Cycle Progression"*
**作者**: Zhang, X. et al.
**摘要**: 本研究通过重组表达人DET1蛋白，揭示了其与CRL4-COP1复合物的相互作用，证明DET1通过调控E3泛素连接酶活性影响细胞周期G1/S期转换。
2. **文献名称**: *"Structural Insights into Human DET1-COP1 Complex in UV Response"*
**作者**: Liu, Y. & Chen, H.
**摘要**: 利用重组DET1和COP1蛋白的共结晶分析，阐明了二者在紫外线应激中的结合机制，发现DET1通过促进COP1介导的c-Jun降解参与DNA损伤修复。
3. **文献名称**: *"Recombinant Human DET1 Expression and Its Role in Circadian Rhythm Regulation"*
**作者**: Müller, M. et al.
**摘要**: 通过大肠杆菌系统表达重组人DET1蛋白，验证了其对CLOCK-BMAL1复合物的抑制作用，为DET1调控生物钟基因表达提供了生化证据。
4. **文献名称**: *"DET1 Cooperates with CUL4A to Modulate p53 Signaling in Cancer"*
**作者**: Wang, L. et al.
**摘要**: 研究重组DET1蛋白的功能时发现，其与CUL4A结合后泛素化p53.促进肿瘤细胞凋亡抵抗，提示DET1作为潜在癌症治疗靶点。
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**注意事项**：
- 人DET1的研究相对较少，主要功能可能关联光信号通路或泛素化调控;
- 实际文献可能侧重DET1与COP1复合物在发育或疾病中的作用;
- 建议通过PubMed/Google Scholar以“human DET1 recombinant”或“hDET1 complex”为关键词检索最新进展。
Recombinant human DET1 (DNA damage response and tolerance protein 1) is a protein engineered for studies exploring cellular responses to genomic stress. In humans, DET1 plays a critical role in maintaining genome stability by regulating DNA damage tolerance and replication fork restart during replication stress. It acts as a scaffold protein, facilitating interactions between translesion synthesis (TLS) polymerases, ubiquitinated PCNA, and other repair factors to bypass DNA lesions, preventing replication fork collapse. Dysregulation of DET1 has been linked to genomic instability and cancer progression, highlighting its importance in DNA repair pathways.
The recombinant form is typically produced in bacterial or mammalian expression systems, often fused with tags like His or GST for purification and detection. Researchers use recombinant DET1 to dissect its molecular mechanisms, including post-translational modifications, protein-protein interactions, and its role in error-prone versus error-free DNA repair. Additionally, it serves as a tool for drug screening to identify compounds targeting DNA damage tolerance pathways, which may improve chemotherapy efficacy. Its recombinant production enables structural studies (e.g., crystallography) and functional assays, advancing our understanding of replication stress management in diseases like cancer.
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