| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DEPDC1 |
| Uniprot No | Q5TB30 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-692aa |
| 氨基酸序列 | MESQGVPPGPYRATKLWNEVTTSFRAGMPLRKHRQHFKKYGNCFTAGEAVDWLYDLLRNNSNFGPEVTRQQTIQLLRKFLKNHVIEDIKGRWGSENVDDNNQLFRFPATSPLKTLPRRYPELRKNNIENFSKDKDSIFKLRNLSRRTPKRHGLHLSQENGEKIKHEIINEDQENAIDNRELSQEDVEEVWRYVILIYLQTILGVPSLEEVINPKQVIPQYIMYNMANTSKRGVVILQNKSDDLPHWVLSAMKCLANWPRSNDMNDPTYVGFERDVFRTIADYFLDLPEPLLTFEYYELFVNILVVCGYITVSDRSSGIHKIQDDPQSSKFLHLNNLNSFKSTECLLLSLLHREKNKEESDSTERLQISNPGFQERCAKKMQLVNLRNRRVSANDIMGGSCHNLIGLSNMHDLSSNSKPRCCSLEGIVDVPGNSSKEASSVFHQSFPNIEGQNNKLFLESKPKQEFLLNLHSEENIQKPFSAGFKRTSTLTVQDQEELCNGKCKSKQLCRSQSLLLRSSTRRNSYINTPVAEIIMKPNVGQGSTSVQTAMESELGESSATINKRLCKSTIELSENSLLPASSMLTGTQSLLQPHLERVAIDALQLCCLLLPPPNRRKLQLLMRMISRMSQNVDMPKLHDAMGTRSLMIHTFSRCVLCCAEEVDLDELLAGRLVSFLMDHHQEILQVPSYLLDC |
| 分子量 | 105.5 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3条与重组人DEPDC1蛋白相关的文献摘要信息举例(*注：以下内容为基于领域研究的虚拟示例，实际文献请通过学术数据库查询)：
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1. **文献名称**：DEPDC1 promotes hepatocellular carcinoma cell proliferation via regulating apoptosis and cell cycle-related proteins
**作者**：Li X, et al.
**摘要**：本研究通过重组人DEPDC1蛋白的体外表达，发现其过表达可抑制肝癌细胞凋亡并加速细胞周期进程，可能通过调控Bcl-2/Bax通路及Cyclin D1表达促进肿瘤发展。
2. **文献名称**：Recombinant DEPDC1 protein enhances chemoresistance in non-small cell lung cancer by activating EGFR signaling
**作者**：Wang Y, et al.
**摘要**：利用重组DEPDC1蛋白处理肺癌细胞，证实其通过激活EGFR/PI3K/AKT通路降低顺铂敏感性，提示DEPDC1可能成为肺癌化疗耐药的新干预靶点。
3. **文献名称**：Structural and functional analysis of human DEPDC1 protein: Implications for cancer immunotherapy
**作者**：Chen H, et al.
**摘要**：首次解析重组人DEPDC1蛋白的三维晶体结构，结合功能实验表明其C端结构域与T细胞抑制受体PD-1存在潜在相互作用，或为免疫检查点治疗提供新方向。
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**建议**：可通过PubMed、Web of Science等平台以“recombinant DEPDC1”或“DEPDC1 oncogenic function”为关键词查找真实文献。近年研究多聚焦于DEPDC1在肿瘤发生、耐药性及免疫微环境调控中的作用。
Recombinant human DEPDC1 (DEP Domain-Containing Protein 1) is a protein encoded by the *DEPDC1* gene located on chromosome 1p34.1. DEPDC1 is evolutionarily conserved and contains characteristic LEXY and DnaQ motifs, though its precise molecular functions remain under investigation. Studies suggest it plays roles in cell proliferation, cycle regulation, and apoptosis resistance, particularly in cancer contexts. Overexpression of DEPDC1 has been observed in multiple malignancies, including hepatocellular carcinoma, bladder cancer, and glioblastoma, correlating with poor prognosis. It is implicated in activating oncogenic pathways like PI3K/AKT/mTOR and modulating p53 signaling, making it a potential therapeutic target.
Recombinant DEPDC1 protein is typically produced using *E. coli* or mammalian expression systems to enable functional studies. Purification strategies often involve affinity tags (e.g., His-tag) followed by chromatography. This recombinant tool aids in exploring DEPDC1's interactome, structural features, and biochemical properties. Researchers utilize it to develop antibodies, study its role in tumorigenesis, and validate CRISPR/Cas9-mediated gene editing. Current research focuses on understanding its dual roles in normal physiology versus cancer progression and evaluating its utility as a diagnostic biomarker or immunotherapeutic antigen.
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