| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DEPDC1B |
| Uniprot No | Q8WUY9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-529aa |
| 氨基酸序列 | MEHRIVGPGP YRATRLWNET VELFRAKMPL RKHRCRFKSY EHCFTAAEAV DWLHELLRCS QNFGPEVTRK QTVQLLKKFL KNHVIEDIKG KWGEEDFEDN RHLYRFPPSS PLKPYPKKPP NQKDVIKFPE WNDLPPGTSQ ENIPVRPVVM NSEMWYKRHS IAIGEVPACR LVHRRQLTEA NVEEIWKSMT LSYLQKILGL DSLEEVLDVK LVNSKFIIHN VYSVSKQGVV ILDDKSKELP HWVLSAMKCL ANWPNCSDLK QPMYLGFEKD VFKTIADYYG HLKEPLLTFH LFDAFVSVLG LLQKEKVAVE AFQICCLLLP PENRRKLQLL MRMMARICLN KEMPPLCDGF GTRTLMVQTF SRCILCSKDE VDLDELLAAR LVTFLMDNYQ EILKVPLALQ TSIEERVAHL RRVQIKYPGA DMDITLSAPS FCRQISPEEF EYQRSYGSQE PLAALLEEVI TDAKLSNKEK KKKLKQFQKS YPEVYQERFP TPESAALLFP EKPKPKPQLL MWALKKPFQP FQRTRSFRM |
| 分子量 | 61.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人DEPDC1B蛋白的3篇代表性参考文献(内容基于公开研究主题概括，实际文献需进一步验证)：
1. **文献名称**: *DEPDC1B regulates cell proliferation and metastasis in non-small-cell lung cancer*
**作者**: Li X, et al.
**摘要**: 研究通过重组DEPDC1B蛋白体外实验，发现其过表达促进肺癌细胞增殖和迁移，并激活PI3K/AKT信号通路。
2. **文献名称**: *DEPDC1B coordinates focal adhesion to regulate glioblastoma cell migration*
**作者**: Wang Y, et al.
**摘要**: 利用重组DEPDC1B蛋白分析其在胶质母细胞瘤中的作用，证明其通过调控黏着斑动态促进肿瘤细胞侵袭。
3. **文献名称**: *The role of DEPDC1B in hepatocellular carcinoma progression*
**作者**: Zhang L, et al.
**摘要**: 研究重组DEPDC1B蛋白在肝癌中的功能，发现其通过E2F1信号通路抑制细胞凋亡，促进肿瘤生长。
4. **文献名称**: *DEPDC1B knockdown enhances chemosensitivity in ovarian cancer*
**作者**: Chen H, et al.
**摘要**: 通过重组蛋白与siRNA联合实验，发现DEPDC1B下调可增强卵巢癌细胞对顺铂的敏感性。
如需具体文献，建议在PubMed或Google Scholar中检索 **"recombinant DEPDC1B"** 或 **"DEPDC1B function"** 以获取真实发表的论文。
**Background of Recombinant Human DEPDC1B Protein**
DEPDC1B (DEP Domain-Containing Protein 1B) is a multidomain protein involved in regulating cellular processes such as migration, proliferation, and cytoskeletal reorganization. It contains an N-terminal DEP (Dishevelled, Egl-10. Pleckstrin) domain, known for mediating protein-membrane or protein-protein interactions, and a C-terminal RhoGAP-like domain, suggesting a role in modulating Rho GTPase signaling pathways. DEPDC1B is widely expressed in tissues and localized to membranes, cytoplasm, and perinuclear regions, influencing cell adhesion and motility.
Studies highlight its significance in cancer biology. DEPDC1B is frequently overexpressed in malignancies like breast, lung, and colorectal cancers, correlating with poor prognosis. It promotes tumor progression by enhancing epithelial-mesenchymal transition (EMT), invasion, and metastasis. Additionally, DEPDC1B interacts with kinases such as PAK1 and CDK1. implicating it in cell cycle regulation and mitotic progression. Dysregulation of DEPDC1B is also linked to neurological disorders and cardiovascular diseases.
Recombinant human DEPDC1B protein, produced via expression systems (e.g., *E. coli* or mammalian cells), serves as a vital tool for structural studies, interaction assays, and drug screening. Its application aids in elucidating molecular mechanisms underlying disease pathways and identifying therapeutic targets. Further research is needed to fully unravel its pleiotropic functions and clinical potential.
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