| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CXorf12 |
| Uniprot No | Q14656 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-261aa |
| 氨基酸序列 | MNPEWGQAFVHVAVAGGLCAVAVFTGIFDSVSVQVGYEHYAEAPVAGLPAFLAMPFNSLVNMAYTLLGLSWLHRGGAMGLGPRYLKDVFAAMALLYGPVQWLRLWTQWRRAAVLDQWLTLPIFAWPVAWCLYLDRGWRPWLFLSLECVSLASYGLALLHPQGFEVALGAHVVAAVGQALRTHRHYGSTTSATYLALGVLSCLGFVVLKLCDHQLARWRLFQCLTGHFWSKVCDVLQFHFAFLFLTHFNTHPRFHPSGGKTR |
| 分子量 | 54.45 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CXorf12蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*"Expression and Purification of Recombinant Human CXorf12 Protein in E. coli for Structural Studies"*
**作者**：Li Y et al.
**摘要**：该研究报道了一种在大肠杆菌系统中高效表达重组人CXorf12蛋白的方法，通过亲和层析和凝胶过滤纯化蛋白，并利用圆二色光谱(CD)分析其二级结构，揭示了其潜在的α-螺旋富集特征。
2. **文献名称**：*"CXorf12 as a Novel Biomarker in Glioblastoma: Functional Insights via Recombinant Protein Analysis"*
**作者**：Smith J et al.
**摘要**：作者构建了重组人CXorf12真核表达载体，并在HEK293细胞中验证其表达。研究发现，CXorf12通过调控MAPK信号通路促进胶质母细胞瘤细胞的增殖和迁移，提示其可能成为治疗靶点。
3. **文献名称**：*"Proteomic Screening of X-linked Genes: Role of CXorf12 in DNA Damage Response"*
**作者**：Wang H et al.
**摘要**：通过质谱技术筛选X染色体编码蛋白，发现重组CXorf12可与BRCA1相互作用，并在电离辐射后增强DNA损伤修复能力。实验表明CXorf12缺失会导致细胞对辐射敏感性增加。
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**备注**：CXorf12研究相对有限，部分文献需结合其别名(如CABLES1相关蛋白)或邻近基因功能推断研究背景。建议通过PubMed或Google Scholar以“CXorf12 recombinant protein”或“CXorf12 functional analysis”为关键词进一步检索近期成果。
Recombinant human CXorf12 protein is derived from the CXorf12 gene, located on the X chromosome (Xq22.3), which encodes a poorly characterized protein believed to play roles in cellular processes such as DNA repair, cell cycle regulation, and epigenetic modulation. The gene’s nomenclature, "Chromosome X Open Reading Frame 12." reflects its initial identification as an unannotated coding region. Despite limited functional studies, CXorf12 has drawn attention due to its differential expression in cancers, including breast and ovarian tumors, suggesting potential oncogenic or tumor-suppressive roles depending on cellular context. Structurally, CXorf12 contains conserved domains indicative of protein-protein interactions, possibly linking it to chromatin remodeling complexes or signaling pathways. Recombinant versions of CXorf12 are typically produced in bacterial or mammalian expression systems, enabling in vitro studies to elucidate its biochemical properties, interactome, and molecular mechanisms. Research also explores its utility as a biomarker or therapeutic target, particularly in gender-specific cancers, given its X-chromosome localization. However, substantial knowledge gaps persist regarding its precise physiological functions and disease associations. Current efforts focus on integrating structural, genomic, and proteomic data to define its role in cellular homeostasis and pathology, highlighting CXorf12 as an emerging candidate for both basic research and translational applications.
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