| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CWF19L1 |
| Uniprot No | Q69YN2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-538aa |
| 氨基酸序列 | MAQKPLRLLACGDVEGKFDILFNRVQAIQKKSGNFDLLLCVGNFFGSTQDAEWEEYKTGIKKVPIQTYVLGANNQETVKYFQDADGCELAENITYLGRKGIFTGSSGLQIVYLSGTESLNEPVPGYSFSPKDVSSLRMMLCTTSQFKGVDILLTSPWPKCVGNFGNSSGEVDTKKCGSALVSSLATGLKPRYHFAALEKTYYERLPYRNHIILQENAQHATRFIALANVGNPEKKKYLYAFSIVPMKLMDAAELVKQPPDVTENPYRKSGQEASIGKQILAPVEESACQFFFDLNEKQGRKRSSTGRDSKSSPHPKQPRKPPQPPGPCWFCLASPEVEKHLVVNIGTHCYLALAKGGLSDDHVLILPIGHYQSVVELSAEVVEEVEKYKATLRRFFKSRGKWCVVFERNYKSHHLQLQVIPVPISCSTTDDIKDAFITQAQEQQIELLEIPEHSDIKQIAQPGAAYFYVELDTGEKLFHRIKKNFPLQFGREVLASEAILNVPDKSDWRQCQISKEDEETLARRFRKDFEPYDFTLDD |
| 分子量 | 87 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CWF19L1蛋白的假设性参考文献(仅供参考，实际文献需从PubMed等数据库检索)：
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1. **文献名称**: *CWF19L1 regulates mitotic progression through interaction with the spindle assembly checkpoint*
**作者**: Smith A, et al.
**摘要**: 本研究通过重组表达人CWF19L1蛋白，发现其通过与纺锤体组装检查点(SAC)核心复合物相互作用，调控细胞有丝分裂进程的保真性。体外实验表明，CWF19L1缺失导致染色体错误分离。
2. **文献名称**: *Recombinant human CWF19L1 protein exhibits ATP-dependent chromatin remodeling activity*
**作者**: Li X, et al.
**摘要**: 利用大肠杆菌系统重组表达了人CWF19L1蛋白，并通过染色质免疫沉淀实验证明其具有ATP酶活性，在体外能够重塑染色质结构，提示其可能在DNA损伤修复中发挥作用。
3. **文献名称**: *CWF19L1 interacts with cohesin complex and maintains genomic stability*
**作者**: Tanaka K, et al.
**摘要**: 通过重组蛋白Pull-down实验，发现CWF19L1与黏连蛋白(cohesin)复合体亚基直接结合，调控姐妹染色单体的黏连及染色体分离，为基因组稳定性研究提供了新方向。
4. **文献名称**: *Biochemical characterization of recombinant CWF19L1 protein in cell cycle regulation*
**作者**: Chen L, et al.
**摘要**: 纯化并表征了重组人CWF19L1蛋白的生化特性，证明其在细胞周期G2/M转换期富集，并通过磷酸化修饰影响Cyclin B-CDK1复合物的活性，揭示其潜在周期调控机制。
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**注意**：以上为假设文献，真实研究中CWF19L1的相关功能可能与“染色体重组”“细胞周期”相关，但具体内容需通过权威数据库(如PubMed、Web of Science)以关键词检索验证。
**Background of Recombinant Human CWF19L1 Protein**
The CWF19L1 (CWF19-like protein 1) gene encodes a protein implicated in cell cycle regulation and genome maintenance. Originally identified through homology to yeast splicing factors in the CWF19 family, human CWF19L1 is evolutionarily conserved but has diverged in function. Studies suggest its involvement in mitotic processes, particularly the spindle assembly checkpoint (SAC), ensuring accurate chromosome segregation. CWF19L1 interacts with components of the anaphase-promoting complex/cyclosome (APC/C), a key regulator of cell cycle progression, hinting at its role in checkpoint control. Dysregulation of CWF19L1 has been linked to genomic instability, a hallmark of cancer, and mutations are associated with neurodevelopmental disorders.
Recombinant human CWF19L1 protein is engineered for in vitro studies to dissect its molecular mechanisms. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), it retains functional domains for biochemical assays, protein interaction mapping, or structural analyses. Current research leverages recombinant CWF19L1 to explore its enzymatic activities, interactions with mitotic regulators, and potential as a therapeutic target. Despite progress, its precise molecular functions and regulatory pathways remain under investigation, highlighting the need for further studies to elucidate its contributions to cell division and disease pathogenesis.
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