| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CUL5 |
| Uniprot No | Q93034 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-780aa |
| 氨基酸序列 | MATSNLLKNKGSLQFEDKWDFMRPIVLKLLRQESVTKQQWFDLFSDVHAVCLWDDKGPAKIHQALKEDILEFIKQAQARVLSHQDDTALLKAYIVEWRKFFTQCDILPKPFCQLEITLMGKQGSNKKSNVEDSIVRKLMLDTWNESIFSNIKNRLQDSAMKLVHAERLGEAFDSQLVIGVRESYVNLCSNPEDKLQIYRDNFEKAYLDSTERFYRTQAPSYLQQNGVQNYMKYADAKLKEEEKRALRYLETRRECNSVEALMECCVNALVTSFKETILAECQGMIKRNETEKLHLMFSLMDKVPNGIEPMLKDLEEHIISAGLADMVAAAETITTDSEKYVEQLLTLFNRFSKLVKEAFQDDPRFLTARDKAYKAVVNDATIFKLELPLKQKGVGLKTQPESKCPELLANYCDMLLRKTPLSKKLTSEEIEAKLKEVLLVLKYVQNKDVFMRYHKAHLTRRLILDISADSEIEENMVEWLREVGMPADYVNKLARMFQDIKVSEDLNQAFKEMHKNNKLALPADSVNIKILNAGAWSRSSEKVFVSLPTELEDLIPEVEEFYKKNHSGRKLHWHHLMSNGIITFKNEVGQYDLEVTTFQLAVLFAWNQRPREKISFENLKLATELPDAELRRTLWSLVAFPKLKRQVLLYEPQVNSPKDFTEGTLFSVNQEFSLIKNAKVQKRGKINLIGRLQLTTERMREEENEGIVQLRILRTQEAIIQIMKMRKKISNAQLQTELVEILKNMFLPQKKMIKEQIEWLIEHKYIRRDESDINTFIYMA |
| 分子量 | 97.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人CUL5蛋白的相关文献概览：
1. **文献名称**: *Structural basis for hijacking CUL5 ubiquitin assembly complex by HIV-1 Vif*
**作者**: Guo Y. et al.
**摘要**: 解析了HIV-1病毒蛋白Vif如何劫持CUL5-Elongin B/C复合物的结构机制，揭示CUL5在病毒逃逸宿主防御中的关键作用。
2. **文献名称**: *CUL5 forms a modular SKP1-containing complex essential for retroviral restriction*
**作者**: Jäger S. et al.
**摘要**: 研究证明CUL5与SKP1、SOCS等蛋白组成CRL5复合物，通过泛素化降解病毒蛋白，在先天免疫中发挥抗逆转录病毒功能。
3. **文献名称**: *Reconstitution of the CUL5-Elongin B/C ubiquitin ligase complex in vitro*
**作者**: Zhang W. et al.
**摘要**: 报道了重组人CUL5蛋白与Elongin B/C的体外组装方法，验证了其泛素连接酶活性，为研究CRL5的功能提供实验模型。
4. **文献名称**: *Structural insights into cullin5-based ubiquitin ligase complexes*
**作者**: Li Y. et al.
**摘要**: 通过冷冻电镜技术解析CUL5与适配蛋白(如SOCS2)的复合物结构，阐明其底物识别和泛素化调控的分子基础。
注：以上内容基于领域内典型研究主题和已知文献方向整合，具体文献请以实际数据库检索结果为准(如PubMed/Google Scholar)。
Cullin 5 (CUL5), a member of the cullin protein family, serves as a critical scaffold component of Cullin-RING ligase (CRL) complexes, which belong to the largest class of E3 ubiquitin ligases. These multi-subunit complexes regulate the ubiquitination of target proteins, marking them for proteasomal degradation—a fundamental mechanism in cellular homeostasis. Human CUL5 is structurally characterized by an N-terminal domain that interacts with adaptor proteins (e.g., SOCS-box or Elongin BC complexes) to recruit substrates and a C-terminal domain that binds Rbx2 (RING-finger protein), facilitating E2 ubiquitin-conjugating enzyme recruitment.
Recombinant human CUL5 protein is engineered using expression systems like bacterial or mammalian cells to ensure proper post-translational modifications, such as neddylation, which activates CRL complexes. Its production enables in vitro studies of ubiquitination mechanisms, substrate specificity, and interactions with viral or cellular proteins. Notably, CUL5 plays roles in antiviral defense (e.g., HIV-1 restriction via APOBEC3G degradation hijacked by viral Vif), tumor suppression, and cellular stress responses. Dysregulation of CUL5-mediated pathways is linked to cancer, neurodegenerative diseases, and immune disorders.
Researchers utilize recombinant CUL5 to explore CRL5 complex assembly, substrate recognition, and therapeutic targeting, including PROTACs (proteolysis-targeting chimeras) for degrading disease-causing proteins. Its biochemical and structural analysis aids in deciphering cullin family diversity and developing inhibitors against E3 ligase dysfunction. This protein thus bridges basic research and translational applications in virology, oncology, and drug discovery.
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