| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CUGBP2 |
| Uniprot No | O95319 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-521aa |
| 氨基酸序列 | MMVEGRLLVPDRINGTANKMNGALDHSDQPDPDAIKMFVGQIPRSWSEKELKELFEPYGAVYQINVLRDRSQNPPQSKGCCFVTFYTRKAALEAQNALHNIKTLPGMHHPIQMKPADSEKSNAVEDRKLFIGMVSKKCNENDIRVMFSPFGQIEECRILRGPDGLSRGCAFVTFSTRAMAQNAIKAMHQSQTMEGCSSPIVVKFADTQKDKEQRRLQQQLAQQMQQLNTATWGNLTGLGGLTPQYLALLQQATSSSNLGAFSGIQQMAGMNALQLQNLATLAAAAAAAQTSATSTNANPLSTTSSALGALTSPVAASTPNSTAGAAMNSLTSLGTLQGLAGATVGLNNINALAGTINTPRSKRLLLPKDNNGMAALNGGLGATGLTNGTAGTMDALTQAYSGIQQYAAAALPTLYSQSLLQQQSAAGSQKEGPEGANLFIYHLPQEFGDQDILQMFMPFGNVISAKVFIDKQTNLSKCFGFVSYDNPVSAQAAIQAMNGFQIGMKRLKVQLKRSKNDSKPY |
| 分子量 | 82.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CUGBP2蛋白的参考文献示例(模拟内容，仅供参考)：
1. **"CUG-BP2 regulates liver regeneration through stabilization of Cyclin D1 mRNA"**
- **作者**: Timchenko NA, et al.
- **摘要**: 研究揭示了CUGBP2在肝脏再生中通过结合Cyclin D1 mRNA的3'非编码区，增强其稳定性并促进肝细胞增殖的分子机制。
2. **"Role of CUGBP2 in myoblast differentiation and muscular dystrophy"**
- **作者**: Singh NK, et al.
- **摘要**: 发现CUGBP2通过调控MEF2A等肌肉分化相关基因的选择性剪接，抑制成肌细胞分化，可能与先天性肌营养不良的病理相关。
3. **"Recombinant CUGBP2 promotes cancer cell proliferation via PI3K/AKT pathway"**
- **作者**: Wang X, et al.
- **摘要**: 研究表明重组CUGBP2蛋白通过激活PI3K/AKT信号通路，增强胃癌细胞的体外增殖能力，提示其促癌作用。
4. **"Expression and functional analysis of recombinant human CUGBP2 in E. coli"**
- **作者**: Zhang Y, et al.
- **摘要**: 描述利用大肠杆菌系统高效表达并纯化重组人CUGBP2蛋白，验证其RNA结合活性及对靶mRNA翻译的调控功能。
*注：以上为模拟文献，实际研究中建议通过PubMed/Google Scholar等平台检索最新论文。*
**Background of Recombinant Human CUGBP2 Protein**
CUGBP2 (CUG-BP Elav-like family member 2), also known as CELF2. is an RNA-binding protein belonging to the CELF family, which regulates post-transcriptional processes such as mRNA splicing, stability, translation, and localization. It contains conserved RNA recognition motifs (RRMs) that mediate interactions with specific RNA sequences. CUGBP2 plays critical roles in tissue development, particularly in muscle and nervous systems, by modulating gene expression networks. Dysregulation of CUGBP2 is linked to diseases including myotonic dystrophy, neurodegenerative disorders, and cancers, where it influences pathological pathways like aberrant splicing or oncogenic signaling.
Recombinant human CUGBP2 protein is engineered for in vitro studies to dissect its molecular functions. Produced via expression systems (e.g., *E. coli* or mammalian cells), it retains RNA-binding activity and is often fused with tags (e.g., His-tag) for purification. Research applications include analyzing RNA-protein interactions, screening therapeutic compounds, and modeling disease mechanisms. Its recombinant form enables precise investigation of CUGBP2's role in RNA metabolism and disease, offering potential for targeted therapies. Current studies focus on its dual roles in cell survival/apoptosis and tissue-specific regulatory networks, highlighting its therapeutic relevance.
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