| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CUGBP1 |
| Uniprot No | Q92879 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-483aa |
| 氨基酸序列 | MNGTLDHPDQPDLDAIKMFVGQVPRTWSEKDLRELFEQYGAVYEINVLRDRSQNPPQSKGCCFVTFYTRKAALEAQNALHNMKVLPGMHHPIQMKPADSEKNNAVEDRKLFIGMISKKCTENDIRVMFSSFGQIEECRILRGPDGLSRGCAFVTFTTRAMAQTAIKAMHQAQTMEGCSSPMVVKFADTQKDKEQKRMAQQLQQQMQQISAASVWGNLAGLNTLGPQYLALLQQTASSGNLNTLSSLHPMGGLNAMQLQNLAALAAAASAAQNTPSGTNALTTSSSPLSVLTSSAGSSPSSSSSNSVNPIASLGALQTLAGATAGLNVGSLAGMAALNGGLGSSGLSNGTGSTMEALTQAYSGIQQYAAAALPTLYNQNLLTQQSIGAAGSQKEGPEGANLFIYHLPQEFGDQDLLQMFMPFGNVVSAKVFIDKQTNLSKCFGFVSYDNPVSAQAAIQSMNGFQIGMKRLKVQLKRSKNDSKPY |
| 分子量 | 78 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CUGBP1蛋白的3篇假设性文献示例(文献信息为虚构，仅供格式参考)：
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1. **文献名称**: *Expression and purification of recombinant human CUGBP1 in E. coli and its RNA-binding activity analysis*
**作者**: Smith J, et al.
**摘要**: 研究报道了在大肠杆菌中高效表达重组人CUGBP1蛋白的优化方法，利用His标签纯化系统获得高纯度蛋白，并通过电泳迁移率实验(EMSA)验证其RNA结合活性，证明其特异性识别CUG重复序列的能力。
2. **文献名称**: *CUGBP1 regulates muscle development through mRNA stability control*
**作者**: Li X, Wang Y, et al.
**摘要**: 研究发现重组CUGBP1蛋白通过结合肌细胞分化相关基因(如MyoD)的mRNA 3'UTR区域调节其稳定性，促进成肌细胞分化，为肌营养不良症等疾病的机制研究提供依据。
3. **文献名称**: *Role of CUGBP1 in alternative splicing of tau exon 10: Implications for Alzheimer’s disease*
**作者**: Garcia R, et al.
**摘要**: 通过体外重组CUGBP1蛋白实验，证实其参与调控tau蛋白外显子10的可变剪接，异常表达可能导致tau病理聚集，提示CUGBP1与阿尔茨海默病的潜在关联。
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注：以上文献为示例性质，实际研究中建议通过PubMed、Web of Science等数据库检索关键词(如“recombinant CUGBP1”、“CUGBP1 function”)获取真实文献。
CUG-BP Elav-like family member 1 (CUGBP1) is an RNA-binding protein critical for post-transcriptional regulation, including mRNA splicing, translation, stability, and decay. It contains three RNA recognition motifs (RRMs) that enable interactions with GU-rich elements in target RNAs. Functionally, CUGBP1 plays roles in developmental processes, muscle differentiation, and cellular stress responses. Dysregulation of CUGBP1 is linked to neurological disorders, myotonic dystrophy type 1 (DM1), and cancers, where altered RNA processing contributes to disease pathology.
Recombinant human CUGBP1 protein is engineered for experimental studies, typically produced in prokaryotic (e.g., E. coli) or eukaryotic expression systems to ensure proper folding and RNA-binding activity. Its recombinant form allows in vitro investigation of RNA-protein interactions, splicing modulation, and translational control mechanisms. Researchers utilize it to elucidate CUGBP1’s role in disease models, screen therapeutic compounds targeting RNA-protein interactions, or analyze its interplay with other regulators like miRNAs or signaling pathways. The protein’s versatility also supports structural studies to map functional domains and mechanistic insights into its dual roles as a translational activator or repressor in context-dependent scenarios.
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