| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CTTNBP2NL |
| Uniprot No | Q9P2B4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-639aa |
| 氨基酸序列 | MNLEKLSKPELLTLFSILEGELEARDLVIEALKAQHRDTFIEERYGKYNISDPLMALQRDFETLKEKNDGEKQPVCTNPLSILKVVMKQCKNMQERMLSQLAAAESRHRKVILDLEEERQRHAQDTAEGDDVTYMLEKERERLTQQLEFEKSQVKKFEKEQKKLSSQLEEERSRHKQLSSMLVLECKKATNKAAEEGQKAGELSLKLEKEKSRVSKLEEELAAERKRGLQTEAQVEKQLSEFDIEREQLRAKLNREENRTKTLKEEMESLKKIVKDLEASHQHSSPNEQLKKPVTVSKGTATEPLMLMSVFCQTESFPAERTHGSNIAKMTNTGLPGPATPAYSYAKTNGHCDPEIQTTRELTAGNNVENQVPPREKSVALAQEKPVENGGCPVGIETPVPMPSPLSSSGSSLSPSSTASSSLTSSPCSSPVLTKRLLGSSASSPGYQSSYQVGINQRFHAARHKFQSQADQDQQASGLQSPPSRDLSPTLIDNSAAKQLARNTVTQVLSRFTSQQGPIKPVSPNSSPFGTDYRNLANTANPRGDTSHSPTPGKVSSPLSPLSPGIKSPTIPRAERGNPPPIPPKKPGLTPSPSATTPLTKTHSQAASLTTAEDLASSCSSNTVVANGKDVELLLPTSS |
| 分子量 | 96.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CTTNBP2NL蛋白的模拟参考文献示例(请注意，以下内容为假设性示例，需根据实际文献验证)：
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1. **文献名称**: *"CTTNBP2NL regulates dendritic spine formation and synaptic function through cortactin interaction"*
**作者**: Chen et al., 2020
**摘要**: 研究通过重组表达人CTTNBP2NL蛋白，揭示了其通过结合cortactin调控树突棘形态发生和突触功能的分子机制，为神经发育障碍提供了潜在机制解释。
2. **文献名称**: *"Recombinant human CTTNBP2NL expression and purification for structural analysis"*
**作者**: Wang et al., 2019
**摘要**: 报道了一种高效重组表达与纯化人CTTNBP2NL蛋白的方法，利用哺乳动物细胞表达系统获得高纯度蛋白，并解析其晶体结构，为后续功能研究奠定基础。
3. **文献名称**: *"CTTNBP2NL deficiency impairs cortical neuronal migration and associates with autism spectrum disorders"*
**作者**: Smith et al., 2021
**摘要**: 利用重组CTTNBP2NL蛋白进行体外神经元迁移实验，发现其缺失导致皮质神经元迁移异常，并通过基因关联分析提出该蛋白与自闭症谱系障碍的潜在联系。
4. **文献名称**: *"Proteomic screening identifies CTTNBP2NL as a novel interactor of mTOR signaling pathway"*
**作者**: Lee et al., 2022
**摘要**: 通过重组蛋白与质谱分析，发现CTTNBP2NL与mTOR通路蛋白相互作用，可能参与细胞生长和代谢调控，提示其在肿瘤中的潜在作用。
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**注意事项**：
- 以上为基于CTTNBP2NL相关研究的常见方向(如神经元发育、蛋白质互作)构造的模拟文献，实际文献需通过PubMed、Google Scholar或专业数据库检索确认。
- 建议检索关键词：**recombinant CTTNBP2NL**, **CTTNBP2NL function**, **CTTNBP2NL structure**。
- 该蛋白可能与自闭症、神经发育及细胞骨架调控相关，可结合这些领域缩小搜索范围。
The recombinant human CTTNBP2NL protein is a engineered variant of the naturally occurring CTTNBP2NL (Cortactin Binding Protein 2 N-Terminal Like) protein, which plays a role in neuronal development and synaptic function. CTTNBP2NL is structurally related to cortactin-binding proteins and contains conserved domains, including coiled-coil regions and cortactin-Src homology 3 (SH3) interaction motifs. It interacts with cortactin, a cytoskeletal protein involved in regulating actin dynamics, particularly in dendritic spines—critical structures for synaptic plasticity in neurons.
Studies suggest CTTNBP2NL modulates dendritic spine maturation and synaptic transmission, linking it to neurodevelopmental processes. Dysregulation of CTTNBP2NL-associated pathways has been implicated in neurological disorders, such as autism spectrum disorders and schizophrenia, highlighting its potential relevance in understanding synaptic dysfunction.
The recombinant form is produced via heterologous expression systems (e.g., E. coli or mammalian cells) to enable functional studies. Its applications include exploring molecular mechanisms of cortactin interactions, actin cytoskeleton remodeling, and synapse formation. Researchers also use it to model disease-associated mutations or screen therapeutic candidates targeting synaptic proteins.
Overall, recombinant CTTNBP2NL serves as a vital tool for dissecting the molecular basis of neuronal connectivity and neurodevelopmental disorders, bridging structural biology with translational neuroscience.
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