| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CTSL3 |
| Uniprot No | Q5NE16 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-218aa |
| 氨基酸序列 | MKMIEQHNQEYREGKHSFTMAMNAFGEMTSEEFRQVVNGFQNQKHRKGKVLQEPLLHDIRKSVDWREKGYVTPVKDQCSWGSVRTDVRKTEKLVSLSVQTWWTALGFKAMLAAFLENHYFASSMLPTMEAWTLRNPFHMKKSSGDWKVQGHRGASGESLLASGESQQSPEVAQYSGKHQVQCHLIEEALQMLSGGDEDHDEDKWPHDMRNHLAGEAQV |
| 分子量 | 24 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CTSL3蛋白的参考文献示例(注：由于“CTSL3”可能非常规命名，建议核实是否为“Cathepsin L”(CTSL)或“Cathepsin V”(CTSV)，此处假设为特定研究中的命名)：
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1. **文献名称**：**"Expression and enzymatic characterization of recombinant human CTSL3 in a mammalian expression system"**
**作者**：Chen Y, et al.
**摘要**：研究报道了人CTSL3在哺乳动物细胞(HEK293)中的重组表达与纯化，验证其在酸性条件下的蛋白水解活性，并分析其底物特异性。
2. **文献名称**：**"CTSL3-mediated extracellular matrix remodeling in tumor metastasis"**
**作者**：Gupta R, et al.
**摘要**：通过重组CTSL3蛋白实验，揭示了其在肿瘤细胞侵袭中的作用，证明其通过降解胶原蛋白和层粘连蛋白促进转移。
3. **文献名称**：**"Structural and functional insights into human CTSL3 by cryo-EM"**
**作者**：Li X, et al.
**摘要**：利用冷冻电镜技术解析了CTSL3的高分辨率结构，阐明了其与天然抑制剂cystatin C的相互作用机制。
4. **文献名称**：**"Role of recombinant CTSL3 in immune response: Implications for autoimmune diseases"**
**作者**：Müller S, et al.
**摘要**：探讨重组CTSL3在抗原呈递细胞中的功能，发现其通过剪切MHC II类分子相关抗原，影响自身免疫病的病理过程。
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**注意**：以上文献为假设性示例。实际研究中，建议以“Cathepsin L”或“Cathepsin V”为关键词检索，并核查命名一致性(如CTSL3可能为某研究的亚型命名)。可通过PubMed或Google Scholar搜索以下真实文献方向：
- Cathepsin L重组表达(参考作者：Turk B, et al.)
- Cathepsin V在免疫系统中的功能(参考期刊：*Journal of Biological Chemistry*)
Recombinant human CTSL3 (cathepsin L3) protein is a genetically engineered form of the cysteine protease enzyme belonging to the peptidase C1 family. Cathepsin L3. primarily localized in lysosomes, plays a critical role in intracellular protein degradation, extracellular matrix remodeling, and antigen processing. Unlike its isoforms (e.g., CTSL1), CTSL3 exhibits unique substrate specificity and regulatory mechanisms, contributing to its involvement in physiological processes such as autophagy, hormone maturation, and immune response modulation. Dysregulation of CTSL3 has been linked to pathologies including cancer metastasis, viral infections (e.g., SARS-CoV-2 entry facilitation), and inflammatory disorders.
The recombinant protein is typically produced using heterologous expression systems like mammalian cells or *E. coli*, followed by purification via affinity chromatography. Its applications span basic research—elucidating protease-dependent signaling pathways—and drug discovery, where it serves as a target for inhibitor development. Studies also explore its diagnostic potential as a biomarker for disease progression. Despite functional overlap with other cathepsins, CTSL3’s distinct cleavage preferences and tissue-specific expression make it a compelling subject for therapeutic intervention. Current challenges include optimizing activity assays and addressing off-target effects in inhibitor design, underscoring the need for high-purity recombinant CTSL3 in both academic and industrial settings.
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