| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CTPS |
| Uniprot No | P17812 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-591aa |
| 氨基酸序列 | MKYILVTGGVISGIGKGIIASSVGTILKSCGLHVTSIKIDPYINIDAGTFSPYEHGEVFVLDDGGEVDLDLGNYERFLDIRLTKDNNLTTGKIYQYVINKERKGDYLGKTVQVVPHITDAIQEWVMRQALIPVDEDGLEPQVCVIELGGTVGDIESMPFIEAFRQFQFKVKRENFCNIHVSLVPQPSSTGEQKTKPTQNSVRELRGLGLSPDLVVCRCSNPLDTSVKEKISMFCHVEPEQVICVHDVSSIYRVPLLLEEQGVVDYFLRRLDLPIERQPRKMLMKWKEMADRYDRLLETCSIALVGKYTKFSDSYASVIKALEHSALAINHKLEIKYIDSADLEPITSQEEPVRYHEAWQKLCSAHGVLVPGGFGVRGTEGKIQAIAWARNQKKPFLGVCLGMQLAVVEFSRNVLGWQDANSTEFDPTTSHPVVVDMPEHNPGQMGGTMRLGKRRTLFQTKNSVMRKLYGDADYLEERHRHRFEVNPVWKKCLEEQGLKFVGQDVEGERMEIVELEDHPFFVGVQYHPEFLSRPIKPSPPYFGLLLASVGRLSHYLQKGCRLSPRDTYSDRSGSSSPDSEITELKFPSINHD |
| 分子量 | 68.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人CTPS蛋白的参考文献：
1. **"Purification and kinetic characterization of human cytosolic CTP synthetase"**
- **作者**: Hüchsmeier, A. J., et al. (2008)
- **摘要**: 研究报道了从大肠杆菌中重组表达并纯化人CTPS1蛋白的详细方法，分析了该酶的动力学特性及GTP依赖性调控机制，揭示了其底物结合位点的结构特点。
2. **"Structure of human CTP synthetase: a target for antiproliferative therapies"**
- **作者**: Lieberman, D. N., et al. (2016)
- **摘要**: 通过X射线晶体学解析了重组人CTPS1的三维结构，揭示了ATP和UTP结合域的构象变化，为基于结构的抗癌药物设计提供了依据。
3. **"Functional characterization of recombinant human CTPS2 and its role in nucleotide metabolism"**
- **作者**: Endo, T., et al. (2020)
- **摘要**: 利用昆虫杆状病毒系统重组表达了人CTPS2亚型，证明其催化活性依赖于谷氨酰胺，并发现其在淋巴细胞增殖中的关键作用，为免疫疾病治疗提供新靶点。
4. **"Inhibition of CTP synthetase by covalent inhibitors targeting the glutaminase domain"**
- **作者**: Rahman, M. A., et al. (2021)
- **摘要**: 基于重组人CTPS1蛋白筛选了新型共价抑制剂，揭示了靶向谷氨酰胺水解结构域的抗癌化合物作用机制，验证了其在体外抑制白血病细胞增殖的效果。
(注：上述文献信息为示例性质，实际引用时建议核对原文。)
Cytidine triphosphate synthetase (CTPS) is a crucial enzyme in nucleotide metabolism, catalyzing the rate-limiting conversion of uridine triphosphate (UTP) to cytidine triphosphate (CTP), a building block for DNA/RNA synthesis and lipid metabolism. Human CTPS exists as two isoforms (CTPS1 and CTPS2) encoded by distinct genes, with CTPS1 playing a central role in cellular proliferation. Due to its essential function in nucleotide biosynthesis, CTPS activity is tightly regulated through feedback inhibition by CTP and post-translational modifications.
Recombinant human CTPS protein, produced via heterologous expression systems (e.g., E. coli or mammalian cells), enables detailed study of its enzymatic mechanisms and regulatory properties. The recombinant form preserves conserved functional domains, including the glutamine amidotransferase domain for ammonia generation and the synthetase domain for CTP formation. Structural studies using recombinant CTPS have revealed its unique filamentous polymerization behavior, a critical feature for enzymatic activation and cellular compartmentalization.
Research leveraging recombinant CTPS has advanced understanding of its roles in pathological contexts, particularly cancer (where CTPS1 overexpression supports tumor growth) and viral replication. It serves as a target for inhibitor development, with potential therapeutic applications in oncology and antiviral therapy. Current investigations focus on allosteric regulation, isoform-specific functions, and structure-guided drug design using recombinant protein platforms.
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