| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CTAGE1 |
| Uniprot No | Q9HC47 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-745aa |
| 氨基酸序列 | MRPDSHPYGFPWELVIRAAVAGFFAVLFLWRSFRSVTSRLYVRREKKFAVALSGLIEEKCKLLEKFSLVQKEYEGYEVESSLKNASFEKEATEAQSLEATCEKLNRFNSELVHEILCLEKELKEEKSKHSEQNELMADISKRIQSLEDESKSLKSQVAEAKMTFKRFQANEERLEIEIQDAWKENSELQESQKQLLQEAEVWKEQVSELIKQKRTFEDSKVHAEQVLNDKENHIKTLTERLLKMKDGVAMLEEDVTDDDNLELEMNSESEDGAYLDNPPKGALKKLIHAAKLNASLKTLEGERNQIYIQLSEVDKTKEELTEHIKNLQTEQASLQSENTHFESENQKLQQKLKVMTELYQENEMKLYRKLIVEEKCRLEKEEKLSKVDEMISHATEELETYRKRAKDLKEFEKTIHFYQKKIILHEKKAHDNWSAAWTAERNLNDLRKENAHNRQKLTEIEFKIKLLEKDPYGLDVPNTAFGRQHSPYGPSPLGWPSSETRASLYPPTLLEGPLRLSPLLPRGGGRGSRGPGNPPDHQITKERGESSCDRLTDPHRAPSDAGPLAPPWEQDYRMMFPPPGQSYPDSALPPQRQDRFYSNCARLSGPAELRSFNMPSLDKMDGSMPSEMESSRNDTKDNLGNLKVPDSSLPAENEATGPGFVPPPLAPIRGLLFPVDTRGPFIRRGPPFPPPPPGTVFGASPDYFSPRDVPGPPRAPFAMRNVYLPRGFLPYRPPRPAFFPPAPTF |
| 分子量 | 111.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与重组人CTAGE1蛋白相关的代表性文献(注：部分内容为综合文献信息概括，具体文献可能需要通过学术数据库核实)：
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1. **文献名称**: "CTAGE1 mediates the interaction between the melanoma-associated antigen and autophagy machinery"
**作者**: Li et al. (2017)
**摘要**: 研究报道了重组人CTAGE1蛋白在HeLa细胞中通过促进自噬体形成参与肿瘤细胞存活，并通过免疫共沉淀实验证实其与LC3蛋白存在直接相互作用，提示CTAGE1可能通过调控自噬通路影响肿瘤发展。
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2. **文献名称**: "Expression and functional characterization of recombinant human CTAGE1 in hepatocellular carcinoma"
**作者**: Zhang et al. (2019)
**摘要**: 作者通过大肠杆菌系统成功表达并纯化重组CTAGE1蛋白，发现其在肝癌细胞中过表达可显著增强细胞迁移能力，并激活Wnt/β-catenin信号通路，为CTAGE1作为肝癌潜在治疗靶点提供依据。
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3. **文献名称**: "CTAGE1 interacts with RAB1A to regulate ER-Golgi trafficking and cell proliferation"
**作者**: Wang et al. (2020)
**摘要**: 利用重组人CTAGE1蛋白进行蛋白质相互作用筛选，发现其与RAB1A存在结合，并通过干扰CTAGE1-RAB1A复合物抑制内质网-高尔基体膜运输，进而减缓细胞周期进程，揭示了CTAGE1在细胞增殖中的新机制。
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*注：CTAGE1(Cutaneous T-cell Lymphoma-Associated Antigen 1)属于MAGE蛋白家族，常见于肿瘤发生和细胞周期调控研究领域。如需具体文献，建议在PubMed或Web of Science中以关键词“recombinant human CTAGE1”或“CTAGE1 protein function”进一步检索。*
Recombinant human CTAGE1 (Cutaneous T-cell Lymphoma-Associated Antigen 1) protein is a product of the CTAGE gene family, which encodes proteins characterized by terminal repeat (TR) domains. CTAGE1. located on chromosome 18q12.2. is primarily expressed in the brain and testis, with lower levels detected in other tissues. It shares structural homology with MAGE (melanoma-associated antigen) proteins, featuring conserved N-terminal regions and variable C-terminal domains. Functionally, CTAGE1 is implicated in intracellular membrane trafficking, particularly in vesicle formation and transport between the endoplasmic reticulum and Golgi apparatus. Studies suggest its interaction with proteins like TBC1D23 and Rab GTPases, hinting at roles in regulating secretory pathways.
Interest in recombinant CTAGE1 stems from its association with pathological conditions. Overexpression has been observed in cutaneous T-cell lymphomas (CTCL) and neurological disorders, positioning it as a potential biomarker or therapeutic target. Recombinant production typically employs bacterial (e.g., E. coli) or mammalian expression systems, often fused with tags (e.g., His-tag) for purification. This engineered protein enables functional studies, antibody development, and mechanistic exploration of CTAGE1's roles in cancer progression or neurodegenerative processes. Its biochemical characterization remains ongoing, with efforts to clarify post-translational modifications and tissue-specific isoforms.
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