| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CT45-6 |
| Uniprot No | P0DMU7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-189aa |
| 氨基酸序列 | MTDKTEKVAVDPETVFKRPRECDSPSYQKRQRMALLARKQGAGDSLIAGSAMSKAKKLMTGHAIPPSQLDSQIDDFTGFSKDRMMQKPGSNAPVGGNVTSSFSGDDLECRETASSPKSQQEINADIKRKLVKELRCVGQKYEKIFEMLEGVQGPTAVRKRFFESIIKEAARCMRRDFVKHLKKKLKRMI |
| 分子量 | 47.19 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CT45-6蛋白的示例参考文献(注：以下内容为基于CT45蛋白家族研究的综合示例，实际文献可能需要进一步验证)：
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1. **文献名称**: *Cloning and Expression of Recombinant Human CT45-6 Protein in Prokaryotic System*
**作者**: Zhang L. et al.
**摘要**: 研究报道了通过大肠杆菌表达系统成功克隆和表达重组人CT45-6蛋白，并优化了纯化条件。Western blot和质谱分析证实其正确折叠和高纯度，为后续功能研究提供基础材料。
2. **文献名称**: *Structural Analysis of CT45-6 Reveals a Cancer/Testis Antigen with Kinase-like Domains*
**作者**: Chen R. et al.
**摘要**: 通过生物信息学及X射线晶体学解析了重组CT45-6的三维结构，发现其具有类激酶结构域，可能参与细胞信号转导。体外实验显示该蛋白在多种癌细胞系中异常激活。
3. **文献名称**: *CT45-6 as a Novel Biomarker for Ovarian Cancer: Recombinant Protein-based Serological Detection*
**作者**: Gupta S. et al.
**摘要**: 利用重组CT45-6蛋白开发了一种ELISA检测方法，发现其在卵巢癌患者血清中高表达，且与肿瘤分期相关。研究表明CT45-6可能作为诊断或预后标志物。
4. **文献名称**: *Immunogenicity of Recombinant CT45-6 in Cancer Vaccine Development*
**作者**: Müller J. et al.
**摘要**: 研究评估了重组CT45-6在小鼠模型中的免疫原性，显示其可诱导强烈的T细胞应答，并抑制移植瘤生长。提示其在癌症疫苗开发中的潜在应用。
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提示：实际研究中建议通过PubMed、Web of Science等数据库，用关键词(如"CT45A6"、"Cancer/Testis Antigen")检索最新文献。部分CT45家族研究可参考真实论文(如涉及CT45A1-A11的癌症相关功能)。
The cancer/testis antigen 45 (CT45) family comprises tumor-associated proteins predominantly expressed in malignancies, with limited or absent expression in normal tissues except the testis. CT45-6. a member of this multi-gene family, has garnered attention for its cancer-specific expression pattern, making it a potential biomarker and therapeutic target. CT45 proteins are encoded by genes on chromosome Xq26.3 and exhibit features typical of cancer/testis antigens, including immunogenicity and restricted somatic expression.
Recombinant human CT45-6 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its biochemical and functional properties. Structurally, CT45-6 contains conserved domains implicated in protein-protein interactions and post-translational modifications, though its exact molecular mechanisms remain under investigation. Studies suggest CT45-6 may regulate signaling pathways linked to tumor progression, such as cell proliferation, apoptosis, and metastasis.
Clinically, CT45-6 overexpression correlates with improved prognosis in certain cancers (e.g., ovarian cancer), potentially reflecting its role in enhancing chemo sensitivity or immune recognition. Its tumor-restricted expression positions CT45-6 as a candidate for immunotherapies, including vaccine development or adoptive T-cell therapies targeting CT45-derived epitopes. Recombinant CT45-6 serves as a critical tool for antibody production, functional assays, and mechanistic studies to explore its oncogenic or tumor-suppressive roles. Ongoing research aims to validate its diagnostic, prognostic, and therapeutic utility across diverse cancer types.
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