| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CSTF3 |
| Uniprot No | Q12996 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-717aa |
| 氨基酸序列 | SGDGATEQA AEYVPEKVKK AEKKLEENPY DLDAWSILIR EAQNQPIDKA RKTYERLVAQ FPSSGRFWKL YIEAEIKAKN YDKVEKLFQR CLMKVLHIDL WKCYLSYVRE TKGKLPSYKE KMAQAYDFAL DKIGMEIMSY QIWVDYINFL KGVEAVGSYA ENQRITAVRR VYQRGCVNPM INIEQLWRDY NKYEEGINIH LAKKMIEDRS RDYMNARRVA KEYETVMKGL DRNAPSVPPQ NTPQEAQQVD MWKKYIQWEK SNPLRTEDQT LITKRVMFAY EQCLLVLGHH PDIWYEAAQY LEQSSKLLAE KGDMNNAKLF SDEAANIYER AISTLLKKNM LLYFAYADYE ESRMKYEKVH SIYNRLLAIE DIDPTLVYIQ YMKFARRAEG IKSGRMIFKK AREDTRTRHH VYVTAALMEY YCSKDKSVAF KIFELGLKKY GDIPEYVLAY IDYLSHLNED NNTRVLFERV LTSGSLPPEK SGEIWARFLA FESNIGDLAS ILKVEKRRFT AFKEEYEGKE TALLVDRYKF MDLYPCSASE LKALGYKDVS RAKLAAIIPD PVVAPSIVPV LKDEVDRKPE YPKPDTQQMI PFQPRHLAPP GLHPVPGGVF PVPPAAVVLM KLLPPPICFQ GPFVQVDELM EIFRRCKIPN TVEEAVRIIT GGAPELAVEG NGPVESNAVL TKAVKRPNED SDEDEEKGAV VPPVHDIYRA RQQKRIR |
| 分子量 | 82.9 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CSTF3蛋白的3篇文献概要(示例基于知识库模拟，非真实文献)：
1. **文献名称**：*Recombinant Human CSTF3 Facilitates mRNA Polyadenylation in vitro*
**作者**：Zhang et al., 2018
**摘要**：报道了重组人CSTF3蛋白在原核系统中的高效表达及纯化方法，证明其可在体外增强mRNA多聚腺苷酸化活性，并揭示其与RNA聚合酶II的相互作用机制。
2. **文献名称**：*Structural Insights into CSTF3 Function in Transcription Termination*
**作者**：Smith et al., 2016
**摘要**：通过X射线晶体学解析重组人CSTF3蛋白的3D结构，提出其RNA结合域在转录终止中识别特异性信号序列的分子基础。
3. **文献名称**：*CSTF3 Knockdown Alters Gene Expression via Dysregulated 3’UTR Processing*
**作者**：Lee et al., 2020
**摘要**：利用重组CSTF3蛋白进行功能回补实验，发现其缺失导致多种癌症相关基因的3’UTR加工异常，影响mRNA稳定性和翻译效率。
注：以上内容为模拟生成的示例，实际文献需通过学术数据库(如PubMed)检索确认。
**Background of Recombinant Human CSTF3 Protein**
The Cleavage Stimulation Factor 3 (CSTF3) is a critical subunit of the Cleavage Stimulation Factor (CSTF) complex, which plays a central role in the 3′-end processing of eukaryotic mRNA. This processing step involves the cleavage and polyadenylation of precursor mRNA (pre-mRNA), a mechanism essential for mRNA stability, nuclear export, and translation. CSTF3 directly interacts with other CSTF subunits (CSTF1. CSTF2) and recognizes specific RNA sequence motifs (e.g., GU-rich elements) downstream of the polyadenylation signal, facilitating the assembly of the processing machinery.
Recombinant human CSTF3 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce a purified, functional form of the protein for research applications. It typically retains RNA-binding activity and interacts with partner proteins, making it a valuable tool for studying mRNA processing mechanisms, gene expression regulation, and RNA-protein interactions. Dysregulation of CSTF3 has been implicated in diseases such as cancer and neurological disorders, where aberrant polyadenylation contributes to pathogenesis.
Research utilizing recombinant CSTF3 spans *in vitro* assays (e.g., RNA binding, cleavage assays) and cellular studies to dissect its role in development, disease models, and therapeutic target exploration. Its recombinant form enables precise mechanistic studies, aiding the development of RNA-targeted therapies.
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