| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CRYBB3 |
| Uniprot No | P26998 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-211aa |
| 氨基酸序列 | MAEQHGAPEQ AAAGKSHGDL GGSYKVILYE LENFQGKRCE LSAECPSLTD SLLEKVGSIQ VESGPWLAFE SRAFRGEQFV LEKGDYPRWD AWSNSRDSDS LLSLRPLNID SPHHKLHLFE NPAFSGRKME IVDDDVPSLW AHGFQDRVAS VRAINGTWVG YEFPGYRGRQ YVFERGEYRH WNEWDASQPQ LQSVRRIRDQ KWHKRGRFPS S |
| 分子量 | 24.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CRYBB3蛋白的模拟参考文献示例(基于研究主题的合理推测，建议通过学术数据库验证真实文献)：
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1. **"Expression and aggregation analysis of recombinant human CRYBB3 in vitro"**
*Author: Zhang Y, et al.*
**摘要**：研究报道了利用大肠杆菌系统表达重组人CRYBB3蛋白的优化方法，分析了该蛋白在体外的聚集特性，提示其在白内障发病中可能的致病机制。
2. **"CRYBB3 mutations linked to congenital cataract disrupt protein stability"**
*Author: Sun H, et al.*
**摘要**：通过定点突变技术研究CRYBB3基因突变对蛋白结构稳定性的影响，发现部分突变导致重组蛋白错误折叠，与先天性白内障表型相关。
3. **"Structural insights into human βB3-crystallin and its role in lens transparency"**
*Author: Kumar S, et al.*
**摘要**：利用X射线晶体学解析重组CRYBB3蛋白的三维结构，阐明其与CRYBB2的相互作用对维持晶状体透明性的关键作用。
4. **"CRYBB3 knockout mouse model reveals its essential role in early lens development"**
*Author: Wang X, et al.*
**摘要**：构建CRYBB3基因敲除小鼠模型，证明该蛋白缺失导致胚胎期晶状体纤维细胞凋亡，为研究白内障发生提供体内证据。
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**注意**：以上为模拟文献，实际文献需通过PubMed、Web of Science等平台以关键词"CRYBB3 recombinant protein"或"CRYBB3 crystallin"检索获取。
**Background of Recombinant Human CRYBB3 Protein**
The human β-crystallin B3 (CRYBB3) protein is a member of the βγ-crystallin superfamily, primarily expressed in the eye lens, where it contributes to maintaining lens transparency and refractive properties. As a structural component, CRYBB3 plays a critical role in organizing lens fiber cells and ensuring optical clarity by forming soluble oligomers with other crystallins. Mutations in the *CRYBB3* gene (located on chromosome 22q11.2) are associated with congenital cataracts, highlighting its importance in lens development and homeostasis.
Recombinant CRYBB3 is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce the protein *in vitro* for functional studies. Its recombinant form allows researchers to explore structural dynamics, interactions with other crystallins, and mechanisms underlying cataractogenesis. CRYBB3 contains characteristic Greek key motifs stabilized by disulfide bonds, a feature critical for its stability in the dense lens environment.
Studying recombinant CRYBB3 aids in deciphering how mutations (e.g., p.G165R) disrupt protein folding or solubility, leading to pathological aggregation. This protein also serves as a tool for biochemical assays, antibody production, and potential therapeutic strategies targeting cataract-related pathways. Research on CRYBB3 bridges gaps in understanding inherited ocular disorders and protein aggregation diseases.
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