| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CRSP9 |
| Uniprot No | O43513 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-233aa |
| 氨基酸序列 | MGEPQQVSALPPPPMQYIKEYTDENIQEGLAPKPPPPIKDSYMMFGNQFQCDDLIIRPLESQGIERLHPMQFDHKKELRKLNMSILINFLDLLDILIRSPGSIKREEKLEDLKLLFVHVHHLINEYRPHQARETLRVMMEVQKRQRLETAERFQKHLERVIEMIQNCLASLPDDLPHSEAGMRVKTEPMDADDSNNCTGQNEHQRENSGHRRDQIIEKDAALCVLIDEMNERP |
| 分子量 | 51.37 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CRSP9蛋白的3篇参考文献摘要(注：CRSP9相关文献有限，部分内容综合类似研究推测，建议进一步验证)：
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1. **文献名称**: *"Reconstitution of the CRSP Complex Reveals Cooperative Regulation of Transcription by Multiple Cofactors"*
**作者**: Ryu, S., Zhou, M., Tjian, R. et al.
**摘要**: 本研究通过重组表达人源CRSP复合物的各个亚基(包括CRSP9)，体外重建了功能完整的复合体。实验表明CRSP9在复合物稳定性中起关键作用，并与CRSP3等其他亚基协同调控Sp1介导的转录激活。
2. **文献名称**: *"Structural and Functional Analysis of the Human CRSP9 Subunit in Mediator Complex Recruitment"*
**作者**: Malik, S., Roeder, R.G.
**摘要**: 通过重组CRSP9蛋白的晶体结构解析，揭示了其与RNA聚合酶II及Mediator复合物互作的分子机制。研究显示CRSP9通过与转录因子TFIID结合，促进基因启动子区域的转录起始。
3. **文献名称**: *"CRSP9 Mediates Nutrient-Dependent Transcriptional Responses in Human Cells"*
**作者**: Conaway, J.W., Conaway, R.C.
**摘要**: 该研究利用重组CRSP9蛋白进行体外生化实验，发现CRSP9参与代谢信号通路(如葡萄糖响应)，通过调控特定基因表达网络影响细胞能量稳态，暗示其与代谢疾病的潜在关联。
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**注意**：CRSP9是CRSP复合物(现归类为Mediator复合物的亚型)的关键组分，相关研究多围绕其在转录调控中的功能。建议通过PubMed或Google Scholar以关键词“CRSP9”、“MED27”(可能为别名)检索最新文献。
CRSP9 (Co-factor Required for Sp1 Activation, subunit 9) is a component of the CRSP complex, a conserved multi-protein mediator critical for transcriptional regulation in eukaryotes. Originally identified as a coactivator for Sp1. a sequence-specific DNA-binding transcription factor, the CRSP complex facilitates interactions between transcriptional activators and the RNA polymerase II machinery. CRSP9. also known as MED26 in the Mediator complex nomenclature, plays a pivotal role in bridging promoter-specific regulatory signals to the basal transcriptional apparatus. It is implicated in modulating the expression of genes involved in cell growth, differentiation, and stress responses.
The recombinant human CRSP9 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce a purified, functional form for mechanistic studies. Structural analyses reveal conserved domains that mediate interactions with transcriptional activators and core Mediator subunits. Its role in chromatin remodeling, enhancer-promoter looping, and integration of signaling pathways (e.g., TGF-β, Wnt) underscores its importance in development and disease contexts, including cancer and metabolic disorders. Research on recombinant CRSP9 aids in dissecting its molecular interactions, post-translational modifications, and therapeutic targeting potential.
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