| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CRSP8 |
| Uniprot No | Q6P2C8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-70aa |
| 氨基酸序列 | MRNKETLEGREKAFIAHFQDNLHSVNRDLNELERLSNLVGKPSENHPLHNSGLLSLDPVQDKTPLYSQLL |
| 分子量 | 33.44 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CRSP8蛋白的假设性参考文献示例，内容基于相关研究领域的常见方向：
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1. **文献名称**：**"CRSP8 Functions as a Transcriptional Coactivator in Human Cells"**
**作者**：J. Martinez et al.
**摘要**：本研究通过重组人CRSP8蛋白的体外表达与纯化，揭示其作为转录中介体复合物(Mediator Complex)的关键亚基，能够直接结合RNA聚合酶II，调控特定基因启动子的活性。实验表明CRSP8的缺失显著抑制细胞分化相关基因的表达。
2. **文献名称**：**"Structural Characterization of Recombinant Human CRSP8 and Its Role in Wnt/β-Catenin Signaling"**
**作者**：L. Chen et al.
**摘要**：利用X射线晶体学解析了重组人CRSP8的三维结构，发现其N端结构域与β-catenin具有直接互作界面。功能实验证实CRSP8通过增强Wnt通路下游基因的转录，促进结直肠癌细胞增殖。
3. **文献名称**：**"CRSP8 Knockdown Impairs Adipogenesis via Dysregulation of PPARγ"**
**作者**：R. Kim et al.
**摘要**：研究通过重组CRSP8蛋白的过表达及siRNA敲低实验，证明其与核受体PPARγ相互作用，调控脂肪细胞分化相关基因的转录。CRSP8的缺失导致脂肪生成标志物(如aP2)表达显著下降。
4. **文献名称**：**"Development of a CRSP8-Based Gene Therapy Vector for Metabolic Disorders"**
**作者**：T. Anderson et al.
**摘要**：探讨重组CRSP8蛋白在代谢调控中的治疗潜力。动物实验显示，肝脏靶向递送CRSP8可改善糖尿病模型小鼠的胰岛素敏感性，机制涉及葡萄糖代谢相关基因的转录激活。
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**注**：以上文献为假设性示例，旨在反映CRSP8蛋白在转录调控、结构生物学、疾病机制及治疗应用中的潜在研究方向。实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。
Cofactor Required for SP1 activation (CRSP) is a multisubunit transcriptional coactivator complex that mediates enhancer-driven gene expression by facilitating communication between DNA-bound transcription factors and the RNA polymerase II machinery. The human CRSP8 subunit, also known as MED26 (Mediator complex subunit 26), plays a pivotal role in this process as a dynamic adaptor within the Mediator complex. It acts as a molecular switch, interacting with both transcription initiation factors (e.g., TFIID) and elongation components (e.g., super elongation complex), thereby regulating the transition from transcriptional initiation to elongation. Structurally, CRSP8/MED26 contains conserved N-terminal glutamine-rich and C-terminal serine-rich domains critical for its scaffolding functions.
Recombinant human CRSP8 protein is typically produced using bacterial or mammalian expression systems, enabling functional studies on its interactions with transcriptional activators (such as SP1. nuclear receptors) and chromatin-modifying enzymes. Its recombinant form has been instrumental in elucidating mechanisms underlying gene-specific regulation, enhancer-promoter looping, and disease-linked transcriptional dysregulation, particularly in cancers and neurological disorders. Recent research highlights its role in recruiting super elongation complexes to control rapid transcriptional responses, making it a potential therapeutic target for conditions involving aberrant transcriptional activation.
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