| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CRSP7 |
| Uniprot No | O95402 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-600aa |
| 氨基酸序列 | MTAAPASPQQIRDRLLQAIDPQSNIRNMVAVLEVISSLEKYPITKEALEETRLGKLINDVRKKTKNEELAKRAKKLLRSWQKLIEPAHQHEAALRGLAGATGSANGGAHNCRPEVGAAGPPRSIHDLKSRNDLQRLPGQRLDRLGSRKRRGDQRDLGHPGPPPKVSKASHDPLVPNSSPLPTNGISGSPESFASSLDGSGHAGPEGSRLERDENDKHSGKIPVNAVRPHTSSPGLGKPPGPCLQPKASVLQQLDRVDETPGPPHPKGPPRCSFSPRNSRHEGSFARQQSLYAPKGSVPSPSPRPQALDATQVPSPLPLAQPSTPPVRRLELLPSAESPVCWLEQPESHQRLAGPGCKAGLSPAEPLLSRAGFSPDSSKADSDAASSGGSDSKKKKRYRPRDYTVNLDGQVAEAGVKPVRLKERKLTFDPMTRQIKPLTQKEPVRADSPVHMEQQSRTELDKQEAKASLQSPFEQTNWKELSRNEIIQSYLSRQSSLLSSSGAQTPGAHHFMSEYLKQEESTRQGARQLHVLVPQSPPTDLPGLTREVTQDDLDRIQASQWPGVNGCQDTQGNWYDWTQCISLDPHGDDGRLNILPYVCLD |
| 分子量 | 91.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CRSP7蛋白的3篇参考文献的示例(注：CRSP7蛋白相关研究较少，以下为假设性示例，实际文献需通过学术数据库核实)：
1. **文献名称**：**"CRSP7 Functions as a Transcriptional Coactivator in Human Cells"**
**作者**：Lee, S. et al.
**摘要**：本研究首次报道了重组人CRSP7蛋白的体外表达与功能验证，证明其通过介导RNA聚合酶II与特定转录因子(如Sp1)的相互作用调控基因表达。
2. **文献名称**：**"Structural Insights into the Mediator Complex Subunit CRSP7"**
**作者**：Zhang, Y. & Weinzierl, R.O.
**摘要**：通过重组表达人CRSP7蛋白并结合X射线晶体学，揭示了其参与中介体复合物(Mediator Complex)形成的关键结构域，强调了其在转录起始中的构象变化。
3. **文献名称**：**"CRSP7 Interacts with p53 to Modulate DNA Damage Response"**
**作者**：Kim, M. et al.
**摘要**：利用重组CRSP7蛋白进行免疫共沉淀实验，发现其与肿瘤抑制蛋白p53直接结合，并参与DNA损伤修复通路的转录调控。
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**注意**：以上为模拟示例，实际研究中CRSP7相关文献较少，建议通过PubMed或Google Scholar以“CRSP7 protein”、“MED27”(可能为别名)或“transcriptional coactivator”为关键词检索最新论文。
Cellular cofactor required for Sp1 transcriptional activation (CRSP) complex, also known as the Mediator complex, is a multi-protein assembly that regulates RNA polymerase II-dependent transcription. CRSP7 (CRSP subunit 7), encoded by the MED7 gene in humans, is a conserved component critical for structural integrity and functional modulation of the Mediator complex. This 27 kDa protein facilitates chromatin remodeling, transcriptional initiation, and interactions between transcription factors and the core transcriptional machinery. Studies suggest CRSP7 plays roles in cell cycle regulation, differentiation, and stress responses by mediating gene-specific activation or repression.
Recombinant human CRSP7 protein is produced via heterologous expression systems (e.g., E. coli or mammalian cells) followed by affinity purification. Its recombinant form enables mechanistic studies of Mediator complex assembly, transcription elongation, and disease-associated mutations. CRSP7 dysregulation has been implicated in cancers, neurological disorders, and developmental defects, making it a focus for therapeutic targeting. Structural analyses of recombinant CRSP7 have revealed its binding interfaces with other Mediator subunits (e.g., MED21 and MED31) and its role in stabilizing the middle module of the complex. Ongoing research aims to dissect its post-translational modifications and context-dependent interactions in gene networks.
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