| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CRSP6 |
| Uniprot No | Q9NVC6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-651aa |
| 氨基酸序列 | MSGVRAVRIS IESACEKQVH EVGLDGTETY LPPLSMSQNL ARLAQRIDFS QGSGSEEEEA AGTEGDAQEW PGAGSSADQD DEEGVVKFQP SLWPWDSVRN NLRSALTEMC VLYDVLSIVR DKKFMTLDPV SQDALPPKQN PQTLQLISKK KSLAGAAQIL LKGAERLTKS VTENQENKLQ RDFNSELLRL RQHWKLRKVG DKILGDLSYR SAGSLFPHHG TFEVIKNTDL DLDKKIPEDY CPLDVQIPSD LEGSAYIKVS IQKQAPDIGD LGTVNLFKRP LPKSKPGSPH WQTKLEAAQN VLLCKEIFAQ LSREAVQIKS QVPHIVVKNQ IISQPFPSLQ LSISLCHSSN DKKSQKFATE KQCPEDHLYV LEHNLHLLIR EFHKQTLSSI MMPHPASAPF GHKRMRLSGP QAFDKNEINS LQSSEGLLEK IIKQAKHIFL RSRAAATIDS LASRIEDPQI QAHWSNINDV YESSVKVLIT SQGYEQICKS IQLQLNIGVE QIRVVHRDGR VITLSYQEQE LQDFLLSQMS QHQVHAVQQL AKVMGWQVLS FSNHVGLGPI ESIGNASAIT VASPSGDYAI SVRNGPESGS KIMVQFPRNQ CKDLPKSDVL QDNKWSHLRG PFKEVQWNKM EGRNFVYKME LLMSALSPCL L |
| 分子量 | 72.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CRSP6蛋白的参考文献示例(均为假设性文献，供参考)：
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1. **文献名称**：*Cloning and Functional Characterization of Recombinant Human CRSP6 Protein*
**作者**：Zhang Y., et al.
**摘要**：本研究成功克隆了人CRSP6基因，并通过大肠杆菌表达系统实现其重组蛋白的高效表达与纯化。Western blot和质谱分析验证了蛋白的正确性。功能实验表明，CRSP6可与转录因子Sp1结合，显著增强其介导的基因启动子活性，提示其在转录调控中的潜在作用。
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2. **文献名称**：*CRSP6 as a Novel Coactivator in Transcriptional Regulation: Structural Insights*
**作者**：Li X., Wang T., et al.
**摘要**：通过免疫共沉淀和荧光共振能量转移(FRET)技术，本研究揭示了重组人CRSP6蛋白与转录复合物Mediator亚基的动态互作。结构分析显示，CRSP6的C端结构域为关键功能区域，其缺失显著降低靶基因的转录激活能力，为相关疾病机制研究提供线索。
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3. **文献名称**：*Overexpression of Recombinant CRSP6 Promotes Tumor Cell Proliferation in Hepatocellular Carcinoma*
**作者**：Chen L., et al.
**摘要**：该研究构建了CRSP6的重组表达载体并转染肝癌细胞系，发现CRSP6过表达显著促进细胞增殖及迁移。RNA-seq分析提示其可能通过调控Wnt/β-catenin通路发挥作用，提示CRSP6或成为肝癌治疗的潜在靶点。
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4. **文献名称**：*Development of a CRSP6-Specific Monoclonal Antibody Using Recombinant Protein*
**作者**：Liu H., et al.
**摘要**：利用重组人CRSP6蛋白免疫小鼠，成功制备了高特异性和亲和力的单克隆抗体。该抗体在免疫组化及染色质免疫沉淀(ChIP)中效果优异，为后续CRSP6在组织分布及分子机制研究提供了关键工具。
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(注：上述文献为虚构示例，实际研究中需查阅真实数据库如PubMed、Web of Science获取权威信息。)
Recombinant human CRSP6 (Co-factor Required for SP1 activation) protein is a key component of the transcriptional regulatory machinery, playing a role in mediating gene expression by facilitating interactions between transcription factors and the basal transcriptional apparatus. CRSP6. also known as MED26 in the Mediator complex, belongs to a conserved family of coactivators essential for RNA polymerase II-dependent transcription. The Mediator complex acts as a molecular bridge, linking sequence-specific DNA-binding activators (e.g., SP1. nuclear receptors) to the general transcription machinery, thereby modulating chromatin structure and transcription initiation.
CRSP6/MED26 is particularly notable for its involvement in regulating genes associated with cellular differentiation, proliferation, and stress responses. Structurally, it contains conserved domains enabling interactions with transcriptional activators and other Mediator subunits. Recombinant CRSP6 is typically produced using expression systems like *E. coli* or mammalian cells, followed by purification for functional studies. Its applications span investigating transcriptional mechanisms, disease-associated dysregulation (e.g., cancers, neurological disorders), and screening for therapeutic compounds targeting transcriptional aberrations. Research on CRSP6 also explores its context-dependent roles in signal integration, histone modification, and enhancer-promoter communication, offering insights into precision therapies for transcription-related pathologies.
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