| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CRNKL1 |
| Uniprot No | Q9BZJ0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-848aa |
| 氨基酸序列 | MTATVENLTFQKDTLGNAVDKNTSRLELRSYSLAGRHGSTEPLVLAWSSQFRRLTWGCALDALHRSPCVAASQHGVTHLIRSSRTPHSTRCRKEDAQPGHHGNGAASVTAQARGQRSVLQVPLPVPRSCLFSESFVVSVSSQSRFLASVPGTGVQRSTAADMAASTAAGKQRIPKVAKVKNKAPAEVQITAEQLLREAKERELELLPPPPQQKITDEEELNDYKLRKRKTFEDNIRKNRTVISNWIKYAQWEESLKEIQRARSIYERALDVDYRNITLWLKYAEMEMKNRQVNHARNIWDRAITTLPRVNQFWYKYTYMEEMLGNVAGARQVFERWMEWQPEEQAWHSYINFELRYKEVDRARTIYERFVLVHPDVKNWIKYARFEEKHAYFAHARKVYERAVEFFGDEHMDEHLYVAFAKFEENQKEFERVRVIYKYALDRISKQDAQELFKNYTIFEKKFGDRRGIEDIIVSKRRFQYEEEVKANPHNYDAWFDYLRLVESDAEAEAVREVYERAIANVPPIQEKRHWKRYIYLWINYALYEELEAKDPERTRQVYQASLELIPHKKFTFAKMWILYAQFEIRQKNLSLARRALGTSIGKCPKNKLFKVYIELELQLREFDRCRKLYEKFLEFGPENCTSWIKFAELETILGDIDRARAIYELAISQPRLDMPEVLWKSYIDFEIEQEETERTRNLYRRLLQRTQHVKVWISFAQFELSSGKEGSLTKCRQIYEEANKTMRNCEEKEERLMLLESWRSFEEEFGTASDKERVDKLMPEKVKKRRKVQTDDGSDAGWEEYFDYIFPEDAANQPNLKLLAMAKLWKKQQQEKEDAEHHPDEDVDESES |
| 分子量 | 119.68 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人CRNKL1蛋白的文献概况(注：以下文献信息为模拟生成，仅供格式参考)：
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1. **文献名称**: *CRNKL1 is essential for DNA repair through regulating homologous recombination*
**作者**: Zhang Y. et al. (2018)
**摘要**: 本研究通过重组人CRNKL1蛋白的体外实验发现，CRNKL1通过结合RAD51蛋白增强同源重组修复活性。敲低CRNKL1会导致细胞对电离辐射敏感性增加，证实其在维持基因组稳定性中的关键作用。
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2. **文献名称**: *Overexpression of recombinant human CRNKL1 promotes colorectal cancer metastasis via Wnt/β-catenin signaling*
**作者**: Li H. et al. (2020)
**摘要**: 研究利用重组CRNKL1蛋白在结直肠癌细胞中过表达，发现其通过激活Wnt/β-catenin通路促进EMT(上皮间质转化)，导致细胞迁移和侵袭能力增强，提示CRNKL1可能是癌症治疗的潜在靶点。
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3. **文献名称**: *Structural and functional characterization of recombinant CRNKL1 in telomere maintenance*
**作者**: Smith J.R. et al. (2019)
**摘要**: 通过重组蛋白晶体结构解析，揭示了CRNKL1的N端结构域与端粒酶RNA组分(TERC)的相互作用机制，证明CRNKL1通过调控端粒酶活性影响细胞衰老进程。
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**补充说明**：
CRNKL1(亦称CLASPIN-like蛋白)在真核生物中高度保守，现有研究多集中于其在DNA损伤修复、癌症进展及RNA代谢中的作用，重组蛋白的制备通常借助大肠杆菌或哺乳动物表达系统。实际文献需通过PubMed等数据库检索验证。
CRNKL1 (Crown-like structure kinase-like 1) is a conserved nuclear protein implicated in transcriptional regulation, RNA processing, and DNA repair. It shares homology with the Drosophila melanogaster gene crooked neck (crn), which is essential for embryonic development. In humans, CRNKL1 contains multiple forkhead-associated (FHA) domains that facilitate phospho-dependent protein interactions, particularly in DNA damage response pathways. Studies suggest its involvement in cell cycle control, maintenance of genomic stability, and pre-mRNA splicing through associations with spliceosome components.
Recombinant human CRNKL1 protein is produced via heterologous expression systems (e.g., E. coli or mammalian cells) to enable biochemical and functional analyses. Its recombinant form retains the ability to bind nucleic acids and interact with partners like TOPBP1 and RNA polymerase II, making it valuable for studying transcriptional elongation mechanisms. Aberrant CRNKL1 expression has been observed in cancers, with potential links to tumor progression and chemoresistance. Current research focuses on elucidating its dual roles in RNA metabolism and DNA repair, as well as exploring therapeutic targeting opportunities. The recombinant protein serves as a critical tool for structural studies, enzymatic assays, and screening potential modulators of CRNKL1-dependent pathways.
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