| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CREB3 |
| Uniprot No | O43889 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-371aa |
| 氨基酸序列 | MELELDAGDQDLLAFLLEESGDLGTAPDEAVRAPLDWALPLSEVPSDWEVDDLLCSLLSPPASLNILSSSNPCLVHHDHTYSLPRETVSMDLESESCRKEGTQMTPQHMEELAEQEIARLVLTDEEKSLLEKEGLILPETLPLTKTEEQILKRVRRKIRNKRSAQESRRKKKVYVGGLESRVLKYTAQNMELQNKVQLLEEQNLSLLDQLRKLQAMVIEISNKTSSSSTCILVLLVSFCLLLVPAIYSSDTRGSLPAEHGVLSRQLRALPSEDPYQLELPALQSEVPKDSTHQWLDGSDCVLQAPGNTSCLLHYMPQAPSAEPPLEWPFPDLFSEPLCRGPILPLQANLTRKGGWLPTGSPSVILQDRYSG |
| 分子量 | 67.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CREB3蛋白研究的参考文献示例(均为虚构，仅作参考)：
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1. **文献名称**：**"Expression and Functional Analysis of Recombinant Human CREB3 in E. coli"**
**作者**：Zhang Y. et al. (2020)
**摘要**：本研究利用大肠杆菌系统表达并纯化重组人CREB3蛋白，通过凝胶电泳和Western blot验证其正确折叠。功能实验表明，该重组蛋白能够特异性结合cAMP应答元件(CRE)，并激活下游基因转录，为体外研究CREB3的调控机制提供了工具。
2. **文献名称**：**"Structural Insights into Human CREB3 DNA-Binding Domain by X-ray Crystallography"**
**作者**：Wang L. et al. (2021)
**摘要**：通过X射线晶体学解析了人CREB3蛋白的DNA结合域结构(2.1 Å分辨率)，揭示了其与CRE位点结合的关键氨基酸残基。突变实验证实了结构预测的功能位点，为开发靶向CREB3的小分子抑制剂奠定基础。
3. **文献名称**：**"CREB3 Mediates ER Stress-Induced Apoptosis in Hepatocellular Carcinoma"**
**作者**：Li H. et al. (2019)
**摘要**：研究探讨了重组人CREB3在内质网应激(ERS)中的调控作用。实验表明，ERS激活的CREB3通过上调CHOP表达促进肝癌细胞凋亡，提示其在癌症治疗中的潜在应用价值。
4. **文献名称**：**"Interaction of CREB3 with ATF6α Enhances Unfolded Protein Response Signaling"**
**作者**：Chen R. et al. (2022)
**摘要**：利用免疫共沉淀和体外结合实验，发现重组人CREB3与ATF6α直接互作，协同增强未折叠蛋白反应(UPR)下游基因表达。该机制为代谢疾病的分子治疗提供了新靶点。
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**注**：以上文献为示例性质，基于CREB3蛋白的典型研究方向(表达纯化、结构、功能、互作)构造，非真实存在。实际研究中建议通过学术数据库(如PubMed、Web of Science)检索最新文献。
CREB3 (cAMP-responsive element-binding protein 3) is a member of the CREB/ATF family of transcription factors, primarily involved in regulating cellular responses to endoplasmic reticulum (ER) stress. Unlike cytosolic CREB1. CREB3 is an ER-resident transmembrane protein with a luminal N-terminal domain that senses ER stress and a cytoplasmic C-terminal domain containing a basic leucine zipper (bZIP) motif for DNA binding. Under basal conditions, CREB3 remains inactive in the ER membrane. Upon ER stress, it undergoes regulated intramembrane proteolysis (RIP) mediated by Site-1 protease (S1P) and Site-2 protease (S2P), releasing its cytoplasmic domain, which translocates to the nucleus to activate target genes involved in protein folding, secretion, and lipid metabolism.
Recombinant human CREB3 protein is typically produced using mammalian expression systems to preserve post-translational modifications. The purified protein, often tagged with affinity markers (e.g., His or FLAG tags), enables studies on its activation mechanism, interactions with ER stress sensors (e.g., IRE1. PERK), and transcriptional regulation. Research highlights its role in diseases linked to ER dysfunction, including cancer, diabetes, and neurodegenerative disorders. Additionally, recombinant CREB3 aids in screening potential therapeutics targeting ER stress pathways or lipid biosynthesis. Its unique position bridging ER stress and gene regulation makes it a critical focus for understanding cellular adaptation mechanisms and developing precision medicine strategies.
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