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Recombinant Human CPNE8 Protein

  • 中文名: 重组人CPNE8蛋白
  • 别    名: Copine 8; Copine VIII; Copine-8; CPNE8; CPNE8_HUMAN; MGC129645; MGC129646
货号: PA2000-6850
Price: ¥询价
数量:
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产品详情

纯度>90%SDS-PAGE.
种属Human
靶点CPNE8
Uniprot NoQ86YQ8
内毒素< 0.01EU/μg
表达宿主E.coli
表达区间1-564aa
氨基酸序列MDSRYNSTAGIGDLNQLSAAIPATRVEVSVSCRNLLDRDTFSKSDPICVLYVQGVGNKEWREFGRTEVIDNTLNPDFVRKFILDYFFEERENLRFDLYDVDSKSPNLSKHDFLGQVFCTLGEIVGSQGSRLEKPIVGIPGKKCGTIILTAEELNCCRDAVLMQFCANKLDKKDFFGKSDPFLVFYRSNEDGSFTICHKTEVVKNTLNPVWQAFKISVRALCNGDYDRTIKVEVYDWDRDGSHDFIGEFTTSYRELSRGQSQFNVYEVVNPKKKGKKKKYTNSGTVTLLSFLVETEVSFLDYIKGGTQINFTVAIDFTASNGNPAQPTSPHYMNPYQLNAYGMALKAVGEIVQDYDSDKMFPALGFGAKLPPDGRISHEFALNGNPQNPYCDGIEGVMEAYYRSLKSVQLYGPTNFAPVINHVARYASSVKDGSQYFVLLIVTDGVISDMAQTKESIVNASKLPMSIIIVGVGPAEFDAMVELDGDDVRVSSRGKYAERDIVQFVPFRDYIDRSGNHILSMARLAKDVLAEIPEQFLSYMRARGIKPSPAPPPYTPPTHVLQTQI
分子量88.44 kDa
蛋白标签GST-tag at N-terminal
缓冲液0
稳定性 & 储存条件Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt.
Reconstituted protein solution can be stored at 2-8°C for 2-7 days.
Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是关于重组人CPNE8蛋白的3篇参考文献示例(部分信息为示例性概括,建议根据实际文献调整):

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1. **文献名称**: *"Recombinant Human CPNE8 Promotes Neuronal Differentiation via Calcium-Dependent Signaling Pathways"*

**作者**: Smith A et al.

**摘要**: 研究报道了重组人CPNE8蛋白通过激活钙离子依赖的ERK和Akt信号通路,促进小鼠神经前体细胞分化为成熟神经元,提示其在神经再生治疗中的潜在应用。

2. **文献名称**: *"Structural Characterization and Functional Analysis of Recombinant CPNE8 in Cancer Cell Invasion"*

**作者**: Zhang Y et al.

**摘要**: 该研究通过大肠杆菌系统表达重组人CPNE8蛋白,解析其晶体结构,并发现其通过调控细胞外基质黏附抑制肺癌细胞的迁移和侵袭,可能与整合素信号通路相关。

3. **文献名称**: *"Expression and Purification of Recombinant CPNE8 for Studies on Synaptic Vesicle Trafficking"*

**作者**: Lee JH et al.

**摘要**: 作者利用昆虫细胞表达系统制备高纯度重组CPNE8.证实其通过与突触小泡蛋白SNAP-25的相互作用,调节突触囊泡的运输及神经递质释放效率。

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**注意**:以上文献信息为示例,部分内容可能为虚构。建议通过PubMed、Google Scholar或Web of Science等数据库,以关键词“recombinant human CPNE8”或“CPNE8 protein function”检索真实文献。


背景信息

**Background of Recombinant Human CPNE8 Protein**

CPNE8. a member of the copine family (CPNE1-CPNE9), is a calcium-dependent phospholipid-binding protein implicated in membrane trafficking, signaling, and cellular processes. The human CPNE8 gene encodes a protein containing two N-terminal C2 domains, which mediate calcium-sensitive membrane interactions, and a C-terminal A-domain involved in protein-protein interactions. CPNE8 is predominantly expressed in the brain, particularly in the hippocampus and cortical neurons, suggesting roles in neural development, synaptic plasticity, or neurodegenerative pathways. Its precise molecular mechanisms remain unclear, though studies link it to regulating receptor tyrosine kinase signaling and vesicle transport.

Recombinant human CPNE8 protein is engineered using heterologous expression systems (e.g., *E. coli*, mammalian cells) to enable functional studies. Purified via affinity tags (e.g., His-tag), it retains calcium-dependent lipid-binding activity. Research applications include elucidating CPNE8's interactions with membrane phospholipids or signaling partners, its role in neuronal differentiation, and potential associations with neurological disorders. As an understudied copine, recombinant CPNE8 serves as a critical tool to explore its biological significance and therapeutic potential in brain-related pathologies. Current investigations focus on structural analysis, calcium signaling modulation, and disease models to define its cellular functions.


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