| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CPNE7 |
| Uniprot No | Q9UBL6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-558aa |
| 氨基酸序列 | MSAGSERGAAATPGGLPAPCASKVELRLSCRHLLDRDPLTKSDPSVALLQQAQGQWVQVGRTEVVRSSLHPVFSKVFTVDYYFEEVQRLRFEVYDTHGPSGFSCQEDDFLGGMECTLGQIVAQKKVTRPLLLKFGRNAGKSTITVIAEDISGNNGYVELSFRARKLDDKDLFSKSDPFLELYRVNDDQGLQLVYRTEVVKNNLNPVWEAFKVSLSSLCSCEETRPLKCLVWDYDSRGKHDFIGEFSTTFEEMQKAFEEGQAQWDCVNPKYKQKRRSYKNSGVVVLADLKFHRVYSFLDYIMGGCQIHFTVAIDFTASNGDPRNSCSLHYINPYQPNEYLKALVSVGEICQDYDSDKRFSALGFGARIPPKYEVSHDFAINFNPEDDECEGIQGVVEAYQNCLPRVQLYGPTNVAPIISKVARVAAAEESTGKASQYYILLILTDGVVTDMADTREAIVRASRLPMSIIIVGVGNADFTDMQVLDGDDGVLRSPRGEPALRDIVQFVPFRELKNASPAALAKCVLAEVPKQVVEYYSHRGLPPRSLGVPAGEASPGCTP |
| 分子量 | 61.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CPNE7蛋白的3篇代表性文献示例(注：文献为虚拟模拟，实际引用需根据真实研究调整)：
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1. **文献名称**：*"Recombinant CPNE7 Protein Enhances Dental Pulp Stem Cell Differentiation and Dentin Regeneration"*
**作者**：Kim S. et al. (2018)
**摘要概括**：研究通过大肠杆菌表达重组人CPNE7蛋白，证明其能促进牙髓干细胞的成牙本质分化，并激活Wnt/β-catenin信号通路，为牙本质再生治疗提供潜在策略。
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2. **文献名称**：*"Functional Analysis of CPNE7 in Osteoblast Differentiation: Role of Recombinant Protein in Bone Formation"*
**作者**：Lee H. & Choi J. (2019)
**摘要概括**：利用哺乳动物细胞系统表达重组CPNE7.发现其通过调控BMP-2信号通路增强成骨细胞分化，促进体外骨结节形成，提示在骨组织工程中的应用价值。
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3. **文献名称**：*"CPNE7 as a Novel Biomarker in Oral Squamous Cell Carcinoma: Recombinant Protein-Based Clinical Validation"*
**作者**：Oh J. et al. (2021)
**摘要概括**：通过重组CPNE7蛋白的免疫组化分析，发现其在口腔鳞癌组织中高表达，并与肿瘤侵袭性相关，提出其作为诊断或预后的潜在生物标志物。
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(注：如需实际文献，建议通过PubMed、Google Scholar等平台以“recombinant CPNE7”或“CPNE7 protein function”为关键词检索最新研究。)
Copine-7 (CPNE7), a member of the copine protein family, is a calcium-dependent phospholipid-binding protein implicated in membrane trafficking, signal transduction, and cell differentiation. The human CPNE7 gene encodes a ~60 kDa protein containing two N-terminal C2 domains that mediate calcium/membrane interactions and a C-terminal A-domain linked to protein binding. CPNE7 is highly expressed in epithelial and mesenchymal tissues, particularly during tooth development, where it regulates odontoblast differentiation and dentin formation by modulating BMP/TGF-β and Wnt/β-catenin pathways. Recent studies highlight its therapeutic potential in regenerative dentistry, as recombinant human CPNE7 (rhCPNE7) stimulates dentin-pulp complex repair and promotes dental stem cell mineralization. Beyond dental biology, CPNE7 shows tumor-suppressive roles in cancers by inhibiting epithelial-mesenchymal transition and PI3K/AKT signaling. Produced via E. coli or mammalian expression systems, rhCPNE7 retains bioactivity for functional assays and preclinical applications. Ongoing research explores its role in neuronal regeneration, bone remodeling, and as a biomarker for tissue-specific pathologies, positioning CPNE7 as a multifunctional regulator of cell adhesion, differentiation, and tissue repair.
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