| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CPNE4 |
| Uniprot No | Q96A23 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-557aa |
| 氨基酸序列 | MKKMSNIYESAANTLGIFNSPCLTKVELRVACKGISDRDALSKPDPCVILKMQSHGQWFEVDRTEVIRTCINPVYSKLFTVDFYFEEVQRLRFEVHDISSNHNGLKEADFLGGMECTLGQIVSQRKLSKSLLKHGNTAGKSSITVIAEELSGNDDYVELAFNARKLDDKDFFSKSDPFLEIFRMNDDATQQLVHRTEVVMNNLSPAWKSFKVSVNSLCSGDPDRRLKCIVWDWDSNGKHDFIGEFTSTFKEMRGAMEGKQVQWECINPKYKAKKKNYKNSGTVILNLCKIHKMHSFLDYIMGGCQIQFTVAIDFTASNGDPRNSCSLHYIHPYQPNEYLKALVAVGEICQDYDSDKMFPAFGFGARIPPEYTVSHDFAINFNEDNPECAGIQGVVEAYQSCLPKLQLYGPTNIAPIIQKVAKSASEETNTKEASQYFILLILTDGVITDMADTREAIVHASHLPMSVIIVGVGNADFSDMQMLDGDDGILRSPKGEPVLRDIVQFVPFRNFKHASPAALAKSVLAEVPNQVVDYYNGKGIKPKCSSEMYESSRTLAP |
| 分子量 | 88.8 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人CPNE4蛋白的假设参考文献示例(基于典型研究方向模拟，非真实文献)：
1. **《Functional characterization of recombinant human CPNE4 in cancer cell proliferation》**
- **作者**: Xu et al.
- **摘要**: 研究通过原核系统表达并纯化重组人CPNE4蛋白，发现其在肺癌细胞中显著促进增殖，可能通过调控MAPK信号通路发挥作用。
2. **《CPNE4 as a novel biomarker: Recombinant protein production and validation in serum samples》**
- **作者**: Li et al.
- **摘要**: 利用哺乳动物表达系统制备高纯度重组CPNE4蛋白，结合ELISA分析显示其在结直肠癌患者血清中高表达，提示其作为诊断标志物的潜力。
3. **《Structural insights into CPNE4-calcium interaction using recombinant protein mutants》**
- **作者**: Zhang & Wang
- **摘要**: 通过重组CPNE4蛋白的突变体构建，揭示其钙离子结合结构域(vWA结构域)在介导膜结合中的关键作用，为靶向治疗提供依据。
4. **《Role of recombinant CPNE4 in neuronal apoptosis and Alzheimer's disease models》**
- **作者**: Chen et al.
- **摘要**: 在HEK293细胞中表达重组CPNE4.发现其过表达加剧β-淀粉样蛋白诱导的神经元凋亡，可能与阿尔茨海默病病理相关。
注：以上文献为假设示例，实际研究需根据具体数据库(如PubMed、Web of Science)检索。真实文献建议使用关键词“recombinant CPNE4”或“CPNE4 protein function”查询。
**Background of Recombinant Human CPNE4 Protein**
CPNE4 (Copine-4) is a calcium-dependent phospholipid-binding protein belonging to the copine family, which is evolutionarily conserved across eukaryotes. These proteins typically feature two N-terminal C2 domains, implicated in membrane interactions, and a C-terminal Von Willebrand Factor A (VWA) domain involved in protein-protein interactions. CPNE4 is ubiquitously expressed, with notable presence in the brain, suggesting roles in neuronal development, synaptic plasticity, and intracellular signaling. Dysregulation of CPNE4 has been linked to pathological processes, including cancer progression, neurodegenerative disorders, and cardiovascular diseases.
Recombinant human CPNE4 is produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable functional and structural studies. Its recombinant form aids in elucidating molecular mechanisms, such as calcium-mediated membrane trafficking, cell adhesion, and transcriptional regulation. Recent studies highlight its potential as a biomarker or therapeutic target, particularly in glioblastoma and Alzheimer’s disease. However, detailed mechanistic insights into CPNE4’s physiological and pathological roles remain limited, necessitating further research to explore its interaction networks and signaling pathways. This protein’s versatility underscores its importance in both basic and translational biomedical research.
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