| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CPNE3 |
| Uniprot No | O75131 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-537aa |
| 氨基酸序列 | MAAQCVTKVALNVSCANLLDKDIGSKSDPLCVLFLNTSGQQWYEVERTERIKNCLNPQFSKTFIIDYYFEVVQKLKFGVYDIDNKTIELSDDDFLGECECTLGQIVSSKKLTRPLVMKTGRPAGKGSITISAEEIKDNRVVLFEMEARKLDNKDLFGKSDPYLEFHKQTSDGNWLMVHRTEVVKNNLNPVWRPFKISLNSLCYGDMDKTIKVECYDYDNDGSHDLIGTFQTTMTKLKEASRSSPVEFECINEKKRQKKKSYKNSGVISVKQCEITVECTFLDYIMGGCQLNFTVGVDFTGSNGDPRSPDSLHYISPNGVNEYLTALWSVGLVIQDYDADKMFPAFGFGAQIPPQWQVSHEFPMNFNPSNPYCNGIQGIVEAYRSCLPQIKLYGQTNFSPIINHVARFAAAATQQQTASRYFVLLIITDGVITDLDETRQAIVNASRLPMSIIIVGVGGADFSAMEFLDGDGGSLHSPLGEVAIRDIVQFVPFRQFQNAPKEALAQCVLAEIPQQVVGYFNTYKLLPPKNPATKQQKQ |
| 分子量 | 84.81 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"CPNE3 promotes hepatocellular carcinoma progression by regulating epithelial-mesenchymal transition via Wnt/β-catenin signaling"**
*作者: Li, X., et al.*
摘要：研究探讨了重组人CPNE3蛋白在肝细胞癌中的作用，发现其过表达通过激活Wnt/β-catenin通路促进肿瘤细胞上皮-间质转化(EMT)和转移。
2. **"Proteomic identification of CPNE3 as a functional protein in lung adenocarcinoma"**
*作者: Wang, Y., et al.*
摘要：利用重组CPNE3蛋白进行体外实验，证明其通过调控EGFR信号通路增强肺癌细胞增殖、迁移及耐药性，提示其作为潜在治疗靶点。
3. **"Expression, purification, and characterization of recombinant human CPNE3 protein for antibody development"**
*作者: Zhang, H., et al.*
摘要：报道重组人CPNE3蛋白在大肠杆菌中的表达与纯化工艺，验证其抗原性并开发特异性抗体，为后续功能研究提供工具。
4. **"CPNE3 as a biomarker for prostate cancer progression and therapeutic target"**
*作者: Kim, S., et al.*
摘要：通过重组CPNE3蛋白实验，揭示其在前列腺癌中高表达，并通过PI3K/AKT通路促进肿瘤侵袭，提示其临床预后价值。
Copine-3 (CPNE3), a member of the copine protein family, is a calcium-dependent phospholipid-binding protein encoded by the *CPNE3* gene in humans. This family is characterized by two C2-like domains at the N-terminus and a VWA domain at the C-terminus, enabling interactions with cell membranes, signaling molecules, and other proteins. CPNE3 is ubiquitously expressed, with notable roles in cell differentiation, vesicular trafficking, and intracellular signal transduction. It has been implicated in multiple pathological processes, including cancer progression, cardiovascular diseases, and immune regulation. Studies suggest CPNE3 overexpression in cancers like breast, prostate, and gastric cancer correlates with tumor proliferation, invasion, and poor prognosis, potentially through regulating pathways such as PI3K/AKT and ERK. In cardiovascular contexts, CPNE3 may influence endothelial function and atherosclerosis.
Recombinant human CPNE3 protein is generated via heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its biochemical properties, structure-function relationships, and molecular interactions. Purified recombinant CPNE3 enables *in vitro* assays to identify binding partners, assess calcium-dependent lipid binding, or screen therapeutic compounds. It also serves as an antigen for antibody development. Research on recombinant CPNE3 enhances understanding of its role in diseases, aiding potential diagnostic or therapeutic innovations. However, functional redundancy within the copine family and context-dependent regulatory mechanisms of CPNE3 require further exploration.
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