| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CPNE2 |
| Uniprot No | Q96FN4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-548aa |
| 氨基酸序列 | MAHIPSGGAPAAGAAPMGPQYCVCKVELSVSGQNLLDRDVTSKSDPFCVLFTENNGRWIEYDRTETAINNLNPAFSKKFVLDYHFEEVQKLKFALFDQDKSSMRLDEHDFLGQFSCSLGTIVSSKKITRPLLLLNDKPAGKGLITIAAQELSDNRVITLSLAGRRLDKKDLFGKSDPFLEFYKPGDDGKWMLVHRTEVIKYTLDPVWKPFTVPLVSLCDGDMEKPIQVMCYDYDNDGGHDFIGEFQTSVSQMCEARDSVPLEFECINPKKQRKKKNYKNSGIIILRSCKINRDYSFLDYILGGCQLMFTVGIDFTASNGNPLDPSSLHYINPMGTNEYLSAIWAVGQIIQDYDSDKMFPALGFGAQLPPDWKVSHEFAINFNPTNPFCSGVDGIAQAYSACLPHIRFYGPTNFSPIVNHVARFAAQATQQRTATQYFILLIITDGVISDMEETRHAVVQASKLPMSIIIVGVGNADFAAMEFLDGDSRMLRSHTGEEAARDIVQFVPFREFRNAAKETLAKAVLAELPQQVVQYFKHKNLPPTNSEPA |
| 分子量 | 87.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人CPNE2蛋白研究的参考文献及其摘要概括：
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1. **文献名称**：*CPNE2 promotes cell proliferation and migration in human lung adenocarcinoma*
**作者**：Li Y, et al.
**摘要**：该研究在大肠杆菌系统中重组表达了人CPNE2蛋白，并验证其通过激活MAPK/ERK信号通路促进肺癌细胞增殖和迁移的作用，表明其可能作为肺癌治疗的潜在靶点。
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2. **文献名称**：*Expression and purification of recombinant human CPNE2 and its interaction with phosphatidylserine*
**作者**：Zhang Q, Wang H.
**摘要**：研究者利用杆状病毒-昆虫细胞系统表达重组CPNE2蛋白，优化纯化流程后发现其与细胞膜磷脂酰丝氨酸结合的能力，为研究其在膜转运中的生物学功能提供依据。
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3. **文献名称**：*Prognostic value of CPNE2 in hepatocellular carcinoma and its role in drug resistance*
**作者**：Chen L, et al.
**摘要**：通过重组CPNE2蛋白的体外实验，发现其在肝癌组织中高表达，并通过抑制凋亡通路增强肿瘤细胞对化疗药物(如顺铂)的耐药性，提示其临床预后价值。
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**备注**：上述文献为示例性内容，实际引用需以真实发表的论文为准，建议通过PubMed、Web of Science或Google Scholar以“CPNE2 recombinant protein”“CPNE2 function”等关键词检索最新研究。
Copine-2 (CPNE2), a member of the calcium-dependent phospholipid-binding copine protein family, plays diverse roles in cellular processes such as membrane trafficking, signal transduction, and cytoskeletal organization. Structurally, it contains two N-terminal C2 domains that mediate calcium-sensitive membrane interactions and a C-terminal von Willebrand factor A (VWA) domain implicated in protein-protein interactions. Recombinant human CPNE2 protein is produced using expression systems (e.g., *E. coli* or mammalian cells) to enable functional studies in vitro.
CPNE2 is ubiquitously expressed and linked to cell adhesion, migration, and apoptosis regulation. It interacts with signaling pathways like Wnt/β-catenin and EGFR, influencing cell proliferation and differentiation. Aberrant CPNE2 expression is associated with cancers, including breast, liver, and lung tumors, where it may enhance metastasis, chemotherapy resistance, or immune evasion. In neurological contexts, CPNE2 modulates synaptic plasticity and neuronal survival, potentially impacting neurodegenerative diseases.
Research on recombinant CPNE2 focuses on elucidating its role in tumorigenesis, biomarker potential, and therapeutic targeting. Its calcium-dependent lipid-binding properties also make it a candidate for studying membrane dynamics. Ongoing efforts aim to map its interactome and validate mechanistic contributions to disease pathways.
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