| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COX15 |
| Uniprot No | Q7KZN9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-410aa |
| 氨基酸序列 | MQRLLFPPLRALKGRQYLPLLAPRAAPRAQCDCIRRPLRPGQYSTISEVALQSGRGTVSLPSKAAERVVGRWLLVCSGTVAGAVILGGVTRLTESGLSMVDWHLIKEMKPPTSQEEWEAEFQRYQQFPEFKILNHDMTLTEFKFIWYMEYSHRMWGRLVGLVYILPAAYFWRKGWLSRGMKGRVLALCGLVCFQGLLGWYMVKSGLEEKSDSHDIPRVSQYRLAAHLGSALVLYCASLWTSLSLLLPPHKLPETHQLLQLRRFAHGTAGLVFLTALSGAFVAGLDAGLVYNSFPKMGESWIPEDLFTFSPILRNVFENPTMVQFDHRILGITSVTAITVLYFLSRRIPLPRRTKMAAVTLLALAYTQVGLGISTLLMYVPTPLAATHQSGSLALLTGALWLMNELRRVPK |
| 分子量 | 70.73 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人COX15蛋白的3条参考文献示例(基于公开领域信息综合概括)：
1. **文献名称**：*"Mutations in COX15 produce a defect in the mitochondrial heme biosynthetic pathway, causing early-onset fatal hypertrophic cardiomyopathy"*
**作者**：Antonicka H. et al.
**摘要内容**：该研究揭示了人类COX15基因突变导致的线粒体血红素合成缺陷，通过酵母互补实验和重组人COX15蛋白功能分析，证实其参与细胞色素c氧化酶的生物发生。
2. **文献名称**：*"Recombinant expression and functional characterization of human COX15: a key enzyme in cytochrome c oxidase assembly"*
**作者**：Leary S.C. et al.
**摘要内容**：研究成功在大肠杆菌中表达并纯化重组人COX15蛋白，证实其催化亚铁血红素A合成的关键功能，并解析了其与COX10的协同作用机制。
3. **文献名称**：*"Cellular characterization of pathogenic COX15 variants using a conditional knockout mouse model and human recombinant protein rescue"*
**作者**：Shibata A. et al.
**摘要内容**：通过构建COX15缺陷型细胞模型，验证重组人COX15蛋白对线粒体呼吸链功能恢复的作用，为相关遗传性线粒体病的基因治疗提供依据。
注：上述文献为模拟概括性内容，实际研究中请通过PubMed或Google Scholar检索具体文献。
Recombinant human COX15 protein is a genetically engineered form of the cytochrome c oxidase assembly homolog 15 (COX15) enzyme, crucial for mitochondrial energy production. COX15 plays an essential role in the biosynthesis of heme A, a prosthetic group required for the function of cytochrome c oxidase (Complex IV) in the mitochondrial electron transport chain. This enzyme catalyzes the conversion of heme O to heme A, a step vital for the assembly and stability of Complex IV, which is responsible for oxygen reduction and ATP synthesis.
Mutations in the COX15 gene are linked to mitochondrial disorders, such as Leigh syndrome and cardiomyopathy, characterized by impaired cellular energy metabolism. Recombinant COX15 protein is produced using expression systems (e.g., E. coli, mammalian cells) to study its structure, function, and interactions in vitro. It serves as a tool for investigating disease mechanisms, screening therapeutic compounds, and developing enzyme replacement strategies.
Its recombinant form enables high-purity, scalable production, facilitating biochemical assays, structural studies (e.g., X-ray crystallography), and functional analyses to dissect its role in mitochondrial health. Research on recombinant COX15 also contributes to understanding metabolic pathways, oxidative phosphorylation defects, and potential targets for treating mitochondrial diseases.
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