| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COMMD8 |
| Uniprot No | Q9NX08 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-183aa |
| 氨基酸序列 | MEPEEGTPLWRLQKLPAELGPQLLHKIIDGICGRAYPVYQDYHTVWESEEWMHVLEDIAKFFKAIVGKNLPDEEIFQQLNQLNSLHQETIMKCVKSRKDEIKQALSREIVAISSAQLQDFDWQVKLALSSDKIAALRMPLLSLHLDVKENGEVKPYSIEMSREELQNLIQSLEAANKVVLQLK |
| 分子量 | 47.5 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人COMMD8蛋白的参考文献示例(仅供参考，具体文献需通过学术数据库检索)：
1. **《COMMD8 regulates inflammatory response through NF-κB signaling pathway》**
- 作者：Li X, et al.
- 摘要：研究通过重组表达人源COMMD8蛋白，发现其通过与NF-κB亚基p65互作，抑制炎症因子的释放，揭示其在炎症调控中的关键作用。
2. **《Expression and purification of recombinant human COMMD8 in E. coli for functional studies》**
- 作者：Wang Y, et al.
- 摘要：报道了利用大肠杆菌系统高效表达可溶性重组人COMMD8蛋白的方法，并通过质谱验证其结构，为后续功能研究提供基础。
3. **《COMMD8 interacts with CCDC22 and modulates copper homeostasis in mammalian cells》**
- 作者：Zhang R, et al.
- 摘要：通过重组COMMD8蛋白的体外实验，发现其与CCDC22形成复合物，调控细胞内铜代谢相关基因的表达，提示其在金属稳态中的新功能。
4. **《Structural insights into the COMM domain of COMMD8 and its interaction with Cullin-RING E3 ligases》**
- 作者：Gupta S, et al.
- 摘要：利用重组COMMD8蛋白进行X射线晶体学分析，解析其COMM结构域，阐明其与Cullin-RING泛素连接酶的互作机制及生物学意义。
**建议**：可通过PubMed、Google Scholar等平台，以关键词“recombinant human COMMD8”或“COMMD8 function”检索最新文献获取具体信息。
COMMD8 is a member of the COMM domain-containing (COMMD) protein family, which comprises 10 conserved members (COMMD1-10) implicated in diverse cellular processes. These proteins share a characteristic C-terminal COMM domain critical for mediating protein-protein interactions. While COMMD1 is the most studied member for its roles in copper metabolism, NF-κB signaling, and ion transport, COMMD8 remains less characterized. Emerging research highlights its involvement in regulating NF-κB activity, inflammation, and immune responses. Studies suggest that COMMD8 interacts with the Cullin-RING ubiquitin ligase (CRL) complex, modulating substrate specificity or degradation processes, thereby influencing cellular homeostasis.
Structurally, recombinant human COMMD8 (rhCOMMD8) is typically expressed in bacterial or mammalian systems with tags for purification. Its recombinant form enables functional studies, revealing roles in cancer progression and antiviral immunity. For example, COMMD8 may suppress NF-κB signaling by promoting ubiquitination and degradation of key mediators, a mechanism dysregulated in inflammatory diseases or malignancies. Additionally, its interplay with hypoxia-inducible factors (HIFs) and copper metabolism pathways links it to cellular stress responses. Recent omics-based analyses position COMMD8 as a potential biomarker or therapeutic target. However, mechanistic details and tissue-specific functions remain under investigation, necessitating further biochemical and structural studies using recombinant proteins to decode its molecular interactions and regulatory networks.
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