| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COMMD10 |
| Uniprot No | Q9Y6G5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-202aa |
| 氨基酸序列 | MAVPAALILRESPSMKKAVSLINAIDTGRFPRLLTRILQKLHLKAESSFSEEEEEKLQAAFSLEKQDLHLVLETISFILEQAVYHNVKPAALQQQLENIHLRQDKAEAFVNTWSSMGQETVEKFRQRILAPCKLETVGWQLNLQMAHSAQAKLKSPQAVLQLGVNNEDSKSLEKVLVEFSHKELFDFYNKLETIQAQLDSLT |
| 分子量 | 49.4 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人COMMD10蛋白的模拟参考文献示例(基于已知研究的合理推测，非真实文献)：
1. **文献名称**：*"Expression and Purification of Recombinant Human COMMD10 in Escherichia coli for Functional Studies"*
**作者**：Li, X. et al.
**摘要**：研究利用大肠杆菌系统高效表达重组人COMMD10蛋白，优化了诱导条件和纯化步骤(使用镍柱亲和层析)，并通过Western blot和质谱验证蛋白完整性，为后续功能研究提供基础。
2. **文献名称**：*"COMMD10 Suppresses Hepatocellular Carcinoma Cell Proliferation by Inhibiting NF-κB Signaling Pathway"*
**作者**：Chen, Y. et al.
**摘要**：通过体外表达重组人COMMD10蛋白，发现其过表达可抑制肝癌细胞增殖，并降低NF-κB通路活性，提示COMMD10在癌症治疗中的潜在作用。
3. **文献名称**：*"Interplay Between COMMD10 and Copper Metabolism: Insights from Recombinant Protein Interaction Assays"*
**作者**：Smith, J. et al.
**摘要**：利用重组COMMD10蛋白进行免疫共沉淀实验，发现其与铜转运蛋白ATP7A存在直接相互作用，揭示了COMMD10在调控细胞铜稳态中的新机制。
4. **文献名称**：*"Recombinant COMMD10 Modulates Endosomal Trafficking via Binding to CCDC22 in Immune Cells"*
**作者**：Wang, R. et al.
**摘要**：通过构建重组COMMD10蛋白并结合CRISPR技术，证实其与CCDC22蛋白相互作用，影响内体运输及巨噬细胞炎症因子的分泌。
**提示**：以上为模拟内容，实际文献需通过PubMed或Google Scholar检索关键词“Recombinant COMMD10”“COMMD10 function”等获取。
Recombinant human COMMD10 (COMM domain-containing protein 10) is a biologically active protein produced through genetic engineering for research and therapeutic applications. COMMD10 belongs to the conserved COMMD family, comprising 10 members (COMMD1-10) implicated in diverse cellular processes, including copper homeostasis, ion transport, NF-κB signaling, and membrane protein trafficking. Specifically, COMMD10 interacts with the COMMD/CCDC22/CCDC93 (CCC) complex to regulate endosomal sorting of transmembrane proteins, such as the copper transporter ATP7A, and modulates cellular copper efflux. Dysregulation of COMMD10 has been linked to pathologies like cancer, cardiovascular diseases, and neurological disorders, given its role in balancing copper levels and mitigating oxidative stress.
The recombinant protein is typically expressed in bacterial, yeast, or mammalian systems, ensuring high purity and functionality for in vitro studies. Its structural features include a conserved N-terminal COMM domain essential for protein-protein interactions and a variable C-terminal region. Researchers utilize recombinant COMMD10 to investigate its molecular interactions, structural dynamics, and regulatory mechanisms in copper metabolism and inflammatory pathways. Additionally, it serves as a tool to explore therapeutic strategies targeting copper-related disorders (e.g., Wilson disease) or malignancies where COMMD10 expression correlates with prognosis. Current studies emphasize its potential as a biomarker or drug target, advancing precision medicine in metal-ion-associated diseases.
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