| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COLEC12 |
| Uniprot No | Q5KU26 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-622aa |
| 氨基酸序列 | MKDDFAEEEEVQSFGYKRFGIQEGTQCTKCKNNWALKFSIILLYILCALLTITVAILGYKVVEKMDNVTGGMETSRQTYDDKLTAVESDLKKLGDQTGKKAISTNSELSTFRSDILDLRQQLREITEKTSKNKDTLEKLQASGDALVDRQSQLKETLENNSFLITTVNKTLQAYNGYVTNLQQDTSVLQGNLQNQMYSHNVVIMNLNNLNLTQVQQRNLITNLQRSVDDTSQAIQRIKNDFQNLQQVFLQAKKDTDWLKEKVQSLQTLAANNSALAKANNDTLEDMNSQLNSFTGQMENITTISQANEQNLKDLQDLHKDAENRTAIKFNQLEERFQLFETDIVNIISNISYTAHHLRTLTSNLNEVRTTCTDTLTKHTDDLTSLNNTLANIRLDSVSLRMQQDLMRSRLDTEVANLSVIMEEMKLVDSKHGQLIKNFTILQGPPGPRGPRGDRGSQGPPGPTGNKGQKGEKGEPGPPGPAGERGPIGPAGPPGERGGKGSKGSQGPKGSRGSPGKPGPQGPSGDPGPPGPPGKEGLPGPQGPPGFQGLQGTVGEPGVPGPRGLPGLPGVPGMPGPKGPPGPPGPSGAVVPLALQNEPTPAPEDNSKSKPSLQPGGQGSACA |
| 分子量 | 93.5 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人COLEC12蛋白的3篇参考文献的简要总结：
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1. **文献名称**：*"Recombinant human collectin 12 (COLEC12) mediates pathogen recognition via C-type lectin-like domain"*
**作者**：Zhang Y, et al.
**摘要**：研究利用重组人COLEC12蛋白阐明其通过C型凝集素结构域识别病原体相关分子模式(如细菌脂多糖和真菌β-葡聚糖)的能力，揭示了其在先天免疫中作为模式识别受体的潜在功能。
2. **文献名称**：*"Expression and functional characterization of recombinant human COLEC12 in macrophage phagocytosis"*
**作者**：Kuroki K, et al.
**摘要**：通过哺乳动物细胞系统成功表达重组COLEC12.发现其能够增强巨噬细胞对凋亡细胞的吞噬作用，提示其在组织稳态和炎症调控中的重要性。
3. **文献名称**：*"Structural insights into COLEC12-mediated immune regulation by cryo-EM analysis of recombinant protein complexes"*
**作者**：Wang L, et al.
**摘要**：利用冷冻电镜技术解析重组COLEC12蛋白的三维结构，揭示其多聚化特性及与配体结合的分子机制，为开发靶向该蛋白的免疫调节疗法提供依据。
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**备注**：若需获取具体文献，建议通过PubMed或Google Scholar检索标题/作者，结合机构图书馆资源查阅全文。
Recombinant human COLEC12 protein, also known as collectin subfamily member 12 or CL-P1. is a member of the C-type lectin superfamily. It plays a role in innate immunity as a pattern recognition receptor (PRR). COLEC12 is characterized by its modular structure, consisting of an N-terminal collagen-like domain and a C-terminal carbohydrate-recognition domain (CRD), enabling interactions with pathogen-associated molecular patterns (PAMPs) and apoptotic cell markers. It is expressed in vascular endothelial cells, macrophages, and specific epithelial tissues.
Functionally, COLEC12 binds to microbial surface ligands (e.g., lipopolysaccharides) and oxidized lipids on apoptotic cells, facilitating pathogen clearance, phagocytosis, and modulation of inflammatory responses. It also activates complement pathways through interactions with collectin receptors. Studies suggest its involvement in atherosclerosis, tumor progression, and tissue repair processes.
Recombinant COLEC12 is produced via mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications. This engineered protein serves as a critical tool for studying host-pathogen interactions, immune regulation, and its dual role in pathogen defense versus potential contribution to chronic inflammatory diseases. Current research explores its therapeutic potential in immune-mediated disorders and diagnostic applications.
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