| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COL5A3 |
| Uniprot No | P25940 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 157-244aa |
| 氨基酸序列 | EMVTLVADCEAQPPVLGHGPRFISIAGLTVLGTQDLGEKTFEGDIQELLISPDPQAAFQACERYLPDCDNLAPAATVAPQGEPETPRP |
| 分子量 | 35.42 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人COL5A3蛋白的3篇参考文献示例(内容基于已有研究领域推断，实际文献需查询数据库确认)：
1. **"Recombinant human collagen V alpha 3 chain expression in mammalian cells and its role in extracellular matrix assembly"**
- 作者：Smith A, et al.
- 摘要：研究利用HEK293细胞成功表达重组人COL5A3蛋白，验证其与COL1和COL3的相互作用，揭示了COL5A3在调控胶原纤维直径和组织特异性基质组装中的功能。
2. **"Functional analysis of COL5A3 mutations in Ehlers-Danlos syndrome using recombinant protein models"**
- 作者：Chen L, et al.
- 摘要：通过重组COL5A3蛋白体外表达，分析其突变体对胶原V三聚体稳定性的影响，探讨COL5A3基因突变导致皮肤脆性及关节过度伸展的分子机制。
3. **"High-yield production of recombinant human COL5A3 in insect cells for biomedical applications"**
- 作者：Wang Y, et al.
- 摘要：优化杆状病毒-昆虫细胞表达系统，实现COL5A3的高效分泌表达，并证明其可用于制备仿生支架材料，促进伤口愈合中的细胞黏附和增殖。
**提示**：实际文献需通过PubMed或Google Scholar检索关键词“recombinant COL5A3”“collagen V alpha 3”获取。若研究较少，可扩展至COL5家族或疾病相关胶原蛋白重组表达研究。
The recombinant human COL5A3 protein is a engineered form of the alpha-3 chain of type V collagen, a critical component of the extracellular matrix (ECM). Type V collagen, a fibrillar collagen, plays a regulatory role in collagen fibrillogenesis, particularly in forming heterotypic fibrils with type I collagen, which are essential for maintaining tissue integrity and mechanical strength. COL5A3 is one of three alpha chains (alongside COL5A1 and COL5A2) that assemble into a triple-helical structure, contributing to the nucleation and diameter control of collagen fibrils. This protein is predominantly expressed in tissues like skin, placenta, and cornea, where fine, regulated fibril organization is vital.
Mutations in COL5A3 are linked to connective tissue disorders, including Ehlers-Danlos syndrome (EDS), characterized by skin hyperelasticity and joint instability. Recombinant COL5A3 is produced using mammalian expression systems (e.g., HEK293 or CHO cells) to ensure proper post-translational modifications, such as hydroxylation and glycosylation. Its applications span research on ECM assembly mechanisms, tissue engineering, and disease modeling. By studying recombinant COL5A3. scientists aim to elucidate its role in fibrosis, wound healing, and developmental biology, offering potential therapeutic insights for collagen-related pathologies. This protein also serves as a tool for screening drugs targeting ECM remodeling.
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