| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | COL16A1 |
| Uniprot No | Q07092 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 117-216aa |
| 氨基酸序列 | WYLFQVTDANGYPQISLEVNSQERSLELRAQGQDGDFVSCIFPVPQLFDLRWHKLMLSVAGRVASVHVDCSSASSQPLGPRRPMRPVGHVFLGLDAEQGK |
| 分子量 | 36.74 KDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人COL16A1蛋白的虚构参考文献示例，简要概括了相关研究内容：
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1. **"高效表达与纯化重组人COL16A1蛋白及其结构表征"**
Smith A, Brown L
研究利用哺乳动物细胞表达系统成功获得重组人COL16A1蛋白，并通过亲和层析纯化。结构分析表明，其C末端非胶原结构域参与细胞外基质组装，为后续功能研究提供基础。
2. **"COL16A1在肿瘤微环境中调控细胞黏附的作用机制"**
Zhang Y, Chen X
通过体外重组COL16A1蛋白实验，发现其能促进癌细胞内皮黏附并激活整合素信号通路，提示其在肿瘤转移中的潜在调控作用。
3. **"重组COL16A1蛋白结构域的功能性研究"**
Jones R, et al.
利用蛋白酶切割技术分离重组COL16A1的不同结构域，发现其N端胶原区域对维持皮肤成纤维细胞机械稳定性具有关键作用。
4. **"COL16A1重组蛋白在干细胞分化中的生物学应用"**
Tanaka K, et al.
研究证明，在培养基中添加重组COL16A1可显著增强间充质干细胞的软骨分化能力，提示其在再生医学中的潜在应用价值。
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以上示例聚焦重组COL16A1蛋白的表达方法、结构功能、疾病关联及生物应用等方向，内容覆盖基础研究与实际应用场景。
Collagen type XVI alpha 1 chain (COL16A1) is a member of the fibril-associated collagens with interrupted triple helices (FACIT) family, which are non-fibrillar collagens involved in stabilizing extracellular matrix (ECM) networks. The COL16A1 protein consists of multiple collagenous domains interspersed with non-collagenous regions, enabling interactions with other ECM components like fibrillar collagens and proteoglycans. It is predominantly expressed in tissues subjected to mechanical stress, such as skin, cornea, and vascular walls, where it contributes to ECM organization, cell adhesion, and tissue integrity. COL16A1 also localizes to basement membrane zones, suggesting roles in cell-ECM crosstalk and tissue remodeling.
Recombinant human COL16A1 protein is produced using expression systems (e.g., mammalian or insect cells) to ensure proper post-translational modifications, such as hydroxylation and glycosylation, critical for its structural and functional properties. This engineered protein serves as a tool to study COL16A1’s interactions with integrins, growth factors, and other ECM molecules, shedding light on its involvement in wound healing, fibrosis, and tumor microenvironment regulation. Dysregulation of COL16A1 has been linked to corneal dystrophies, skin aging, and cancers, where its overexpression may promote metastasis or drug resistance. Research utilizing recombinant COL16A1 aids in exploring its potential as a therapeutic target or diagnostic biomarker for ECM-related pathologies.
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